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modernpace · 3 years

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Loki x Reader Chapter 4

Chapter 4:

Thor led you to a lavish banquet hall. A long wooden table sat at the center of the room and was filled with men and women. They were talking and laughing, telling stories of their adventures. The two of you stopped as he scanned the room. His eyes landed on a beautiful woman standing off to the side. He guides you over to her and bows his head.

"Mother."

She smiled brightly and held her hand out to him. Her golden locks were pinned up, like a halo made of hair. Her gown was a dandelion yellow, with a sheer mesh-like neckline decorated with a mosaic of gems.

You quickly snap out of it and curtsy with your fist over your heart and your head bowed.

"This must be your friend," she said patting his arm.

"Y/n L/n, your majesty," you responded. Thank god Thor briefed you about introductions on your way here...who know what would happen if you were rude to the Queen.

She gently lay a hand on your arm, "Come, let me have a look at you, dear." You stand and look up, but avoid looking at her directly. I must look like an idiot. "The dress looks lovely on you. Compliments you well." She smiled kindly at you. You saw so much of Thor in her face: kind eyes, big smile.

You beamed at her, "I can't thank you enough, your majesty, for allowing me to come to Asgard. It's a beauty like no others."

Nodding she responded, "And as far as my husband is concerned, I'm the one who offered you this opportunity after my son confided in me." She winked at you, and without question, you nodded in agreement. She turned to Thor and laid a hand on his bicep and gave him a sympathetic smile.

They shared a knowing look before she excused herself, "I hope you enjoy the banquet, my dear. Tomorrow we'll discuss your training." Without another word, she left the banquet hall.

"Shall we?"

"Wait." He looked back at you. "What did she mean?"

He nodded, "She's probably going to bring Loki some books. I don't know why she entertains him. He--"

"No," you interrupted, "you know what I mean."

"My father..." you nodded for him to continue. "He doesn't believe Midgardians should be on Asgard."

Your mouth dropped slightly and you muttered, "Well that would've been great to know beforehand"

He gave you an apologetic look and began walking toward a different door. You followed closely behind him. He led you to a throne room. High pillars line the hall on both sides. On the opposite end you entered from, there were no walls, just a beautiful view of Asgard and its city. Standing under an archway, Odin stood and studied his kingdom.

"Father." Odin didn't flinch, or turn or even acknowledge your presence. He just stared out at Asgard. "I present Lady Y/n." You bow with your fist against your heart.

"Have you settled in nicely?" He inquired.

"Um," you instantly got your shit together, forgetting your nerves. "Yes, All-Father. Thank you for you opening your home to me, and allowing me the opportunity to hone my abilities, sir."

Your eyes remained at his feet and he chuckled. "Very well." He turned to his son and with a stern voice advised him, "During her stay on Asgard, she will be your responsibility. You are to ensure her safety, as well as maintain she stay out of trouble."

"Yes, of course, father," Thor responded with certainty.

"My dear," you daringly look up at him and he motions you to stand, "My wife tells me you possess the ability of illusions."

You cursed to yourself. Is it rude to correct the King? "That's definitely one way to put it. I hope to discover the full extent of my abilities so I can help people back home."

He gives a cautious smile, "I hope you remember that with great power, comes great responsibility. Any power can be used for good--or even bad for that matter. Carry that with you during your training." You hummed in understanding and he gestured for you two to head back to the banquet hall. "You may go, enjoy the banquet."

You both thanked him and made your way back to everyone.

"Definitely more intimidating than you said," you joked.

****

Thor had introduced you to his close friends: the Warriors Three, and Lady Sif. Volstagg was loud and hearty, Hogun was serious and silent, and Fandral was extremely polite and a little... flirtatious? Sif was bubbly but serious...definitely more bubbly when drinking.

They had welcomed you, asked you if you had any stories--which you didn't because you never actually went on a legitimate assignment. They still seemed warm and open to you. You were grateful for that.

Volstagg was telling a story about when he had come to Earth a few years back--when Thor had been cast out of Asgard. From Thor's hearty laugh and Sif's questionable smile, you could tell he was a very creative story-teller.

You weren't able to keep up with their high tolerance. And you thought Thor was an experienced drinker. Having all five of them guzzle down their ale, while you're not even done with your second mug. It didn't help that Asgardian alcohol was much more...intense.

Thor somehow lost track of your whereabouts while he and Volstagg were having a drinking contest. You found yourself stumbling into the dungeons, with a newfound sense of confidence. You wanted to confront Loki and his idiot face about his idiot remarks from earlier. You stumbled from cell to cell, forgetting where Loki was.

"You're not Loki," you moved to the next cell, "Ne-neither 're y-you." You looked around confused, the room spun a bit."Wherethehell isthat little--Oh!" You grinned like an idiot as you approached his cell.

He had an amused look on his face and mumbled, "This should be good."

You pointed your finger and narrowed your eyes, "You."

"Yes?"

"You...you..." you forgot what you were going to say. You tried to study his face but he kept swaying. Why was he swaying?

"Not very lady-like for you to be wandering the halls drunk as an idiot on your first night in Asgard."

"W-willyoushutup," You growled as you leaned against the stone beam next to his cell. "You oweme an an ap-apopology." You tried to look fierce and angry...but this was an embarrassing sight. Your hair was frizzy, your dress was disheveled, your eyes were drooping.

He stifled a laugh, never had he thought a mortal would look so funny...stupid, yes, pathetic, yes, but funny? This was a first.

You continued your drunken rant.

"I camehere t-t-to help Muscles. Cuzthat's what friends arefor," you whined and stomped your foot. Your knees felt week and your body was tired but you ignored them. "Imgonna be 'ere for-for, well Idunno how long but" you burped, "I made a promise. And I nev-never break 'em."

You looked directly into his emerald eyes. "Never," you emphasized.

He raised an eyebrow, "Is that so?"

"Shuddup." You looked him over and huffed, "You're such a-an emo."

"What the bloody hell does that mean?" You waved your hand in dismissal.

"A-and a dra-drama queen." You giggle to yourself, imagining Loki like a 1950's actress, prim and dramatic...and so demanding. (I promise this isn't a dig at any actresses, just tryna emphasize Loki is a drama queen).

"You're quite pathetic," he said in a matter-of-fact one. You glared at him. Being done with your conversation you began to regain your balance. You wanted to leave him with one last gift before the night ended.

A malicious grin formed on your face.

Loki raised an eyebrow cautiously. You clumsily rubbed your hands together as your thought of your sweet revenge. He was completely dumbfounded by your strange actions, not knowing what was in store for him. You opened your eyes and giggled devilishly.

"I hope you and your new roommate get acquainted." A low growl from behind startled him. He slowly turned around and he paled when he saw a wolf twice his size standing opposite him. "I would hate to see you turned to dog-chow." Contentedly you turned around and happily stumbled a bit.

"Wait!"

You stopped.

"Wait, you can't kill me. You won't kill me."

You glanced back, "Oh?"

The wolf inched closer, snarling at him.

"Wait wait wait wait."

He pleaded with you. How...out of character for the big bad wolf I heard about, you thought. You chuckled as the wolf growled and pounced. He flinched and turned his body away from it, waiting to be torn to shreds. But it never came.

You burst out laughing and lost your balance. You landed on the stone floor, gasping for air.

Loki looked at you with pure outrage. The wolf stood outside the cell now, circling you protectively.

"You deceived me."

You finally caught your breath. "Oh god, that was good." You finally felt a little more stable and stood up, "Didn't think the God of Mischief would fall for something like that.”

"You'll regret that you mewling quim," he growled like your wolf. You could practically see his aura around him: rageful, vengeful, out-of-his-mind angry. But you shrugged it off. Sweet sweet psychological torture.

"Doubtful. Totally worth it," you quipped shaking your head with a proud smile.

You waved your hand and the wolf dispersed into the air. "Gooooodniiiiight," you sang as you left Loki's cell.

___________

A/N: Hi Everyone! I just finished this chapter at 4 AM!!!!!!!!I clearly don't get enough sleep lmao.I'm gonna work on chapter 5 tomorrow; my plan is to publish it tm but expect it for Monday evening.I hope you enjoy this chapter! Goodnight! (or Goodmorning lol). PLEASE LEAVE FEEDBACK

-Izzy

#avengers #avengers fanfiction #avengers au #the avengers #loki x you #loki #loki laufeyson #loki fanfic #loki x reader #self insert #x reader #fanfic #thor odinson #tony stark #mcu #marvel #wandavision #avengers endgame

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faakeid · 4 years

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First taemin speaks the word Jagi, then Baek says something about dinner at Ji's house. Now Baek said "we are in ilsan" and Taemin asked Ji where he lives. Why Baek and Tae keep playing about Ji's house. The way they look at Ji when they talk about the house. They are teasing Ji all the time and spilling a lot of tea lately. This is the second time that they mentioned Ji's home and he indirectly said no, not to mention it again. But we know whose hometown in Ilsan is

Thank you @bellamarkt for sending the original tweet with the discussion!

Stuff to point out:

How the members who most engage in mocking JI are Tae*in and Baek*yun;

The expression involving them and N*T members are hilarious since they’re so different;

How they seem to be mocking JI and he promptly responds (first denying when TM suggested jagi name and now this looool);

Coincidences

(JI mentions that while talking about KS)

(source from the SS).

My thoughts: well, since other members have their own places, it wouldn’t surprise me if it was the same with JI. But it seems there are more points he doesn’t want address ;)

#im terrible with twitter but im glad about things are being discussed there #until someone comes and fuck everything talking shit #twitter sucks

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diindjariin · 4 years

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Have You Ever Been In Love? (Din Djarin x Reader)

Rating: t+

Word Count: 3.5k

Excerpt: “I like who you are now,” you say, hoping this will ease his pain, if only a little bit. The tilt of his head lets you know he’s smiling.

“And you?” he asks, “Have you ever been in love?”

“Oh yes,” you reply, breathless, and whisper for you in your head so many times you hope he can hear you.

Masterlist for this series

A/N: this is the third time i have tried posting this to get it in the stupid tumblr tags, so my apologies if you’ve seen this a billion times. also, the screencap sucks again. roast me, please. if you’ve been following this series, this takes place right after soft hands, so it is not only the newest fic in the series, it is the newest chronologically. also, i am currently taking suggestions for the name of this series. feel free to help me out! as per usual, this fic had a quick once-over before being published so…………….expect errors. i admit wholeheartedly that i am the worst TM.

“We need money.”

You’re sat cross-legged on Mando’s cot sewing up a hole in one of his undershirts. Normally you wouldn’t bother, because he insists it isn’t necessary, but it’s your favorite; the brown one that’s a bit too small and shows off the muscles in his arms. You look up at him briefly and then return to your sewing, answering him without looking.

“Okay. What’s the plan?”

“I’m going to get in contact with my old partner.” Your whole body stops and the only movement comes from your eyes, which slowly inch their way up his body and rest on the T of his visor.

“Mando –“ you don’t know what else to say. He’s told you about his past, bits and pieces at least, but enough for you to know that anyone he used to be in contact with were not people he should associate with again. He’s proud of himself for leaving that part of his life behind, has told you as much, but now here he is, going back.

“This isn’t up for negotiation,” he doesn’t sound angry, but he sounds sure. “I just want to discuss what to do with you and the kid.”

So, you do. You don’t want him to be alone, but you also have your limits. You have no desire to be involved in anything criminal, you tell him, which prompts him to remind you of the child and that protecting him counts as criminal involvement.

“You could drop us off on Sorgan,” you say casually, stroking the Child’s ears idly. “I’m sure Cara wouldn’t mind the company.”

“I don’t like the idea of the two of you out of my orbit,” he responds. Your breath catches in your throat but he doesn’t seem to parse the deeper meaning of his words. “If you both just stay on this ship you’ll be fine. You’ll never even have to see them. Ok?”

“It seems like you’ve already made up your mind,” you say, turning slightly so you’re no longer facing him and go back to your needlework. The Child seems to sense the shift in your mood and whimpers, burrowing into your lap. Mando sighs and rests his hands on his hips.

“Are you mad at me?”

“Yes,” you respond, “a bit. Why do you ask for my opinion if you’re just going to do what you want anyway?” Mando groans, long and deep, and tilts his head in an attempt to get you to look at him.

“Hey,” he says, but you don’t turn. He repeats himself, leans more into your field of vision. “Hey – look at me.” You do, albeit cautiously. “I care. What you think. But I’m the bounty hunter. I’m gonna do what I think is safest. But I still care how you feel about it.” Now it’s your turn to sigh, nod your head once. Your eyes fall shut and you rub at the space between your eyes.

“I know…” you let your voice trail off as you desperately push against the beginning of a migraine. “I’m sorry for insinuating otherwise.” He nods his head, then turns on his heel and makes his way to the cockpit. You allow your upper body to fall backwards on his cot, legs still crossed, the slight bounce sending the child a few inches into the air. He falls back between your legs and giggles, crawls up your body so he’s snuggled under your chin. You close your eyes and sigh, wondering how the hell you’re supposed to be okay with this. You throw your arm over your eyes and push the thoughts from your mind.

———————————————————————————————————-

“Why can’t you remove your helmet?”

Mando is indulging you. He knows how much you love the stars, the sounds of nature. There’s nothing pressing to attend to and so you park on a mostly uninhabited planet, open the hatch, and watch the stars. Not speaking but enjoying the other’s presence, nonetheless. Mando’s manspreading, something you tease him for mercilessly but love when he does it. It means he’s comfortable. He’s got his legs spread wide, leant back on his elbows. He turns his head as if to look at you out of his peripheral vision. Then he goes back to looking at the stars.

“It’s… my religion,” he says. You think he’s going to stop here but, to your amazement, he speaks the most he ever has to you in one breath. “It wasn’t always this way, I’ve heard. But my people were hunted, had to go into hiding. It… made sense not to remove our helmets. For safety. And then it became about something else.” He pauses for a moment, searching for words, then continues. “It bothered me at first. But I got used to it, after a while. Sacrifices are necessary in life.”

“Yes, but –“ you say, then stop yourself, not wanting to offend him. The Mandalorian tilts his head and turns toward you, his equivalent of an eyebrow quirk. “Your life shouldn’t be sacrificed.” He doesn’t have a response for that, but he does shift that much closer, enough that the length of your arms tough. You think he might agree.

“One more time,” he says, “what’s the story?”

“If someone gets in, I’m your slave,” you barely get the words out, cringing at the thought of being owned by someone. “I don’t like saying that. You’d never do that.” Mando’s head ticks up quick and he looks like he wants to say something to that but changes his mind.

“It’s the only thing that will make sense to these assholes without giving away that we have the kid. Okay? But you shouldn’t have to worry about anyone.” His fingers trace your cheekbone. He likes the way the leather looks against your skin. Then he’s turning, making his way over to the hatch. “Stay away from the hatch,” he says before lifting it, “and don’t make any noise.” Then he raises the door, shuts it behind him, and is gone.

———————————————————————————————————-

“Have you ever been kissed?”

This is going to become a thing between the two of you, you know. Nights spent on barren planets, swapping secrets. It’s easier to get him to open up first, so that he has a reference point for how personal he can get with you.

“No,” he responds. He stares at his feet instead of up at the sky, which just won’t do. You sit up on your knees and pitch yourself forward, grabbing his hands in yours.

“Someday, then,” you say softly, big eyes staring up at him like you can see him. He doesn’t respond, but he does cup your cheek in his hand, bare thumb brushing over your bottom lip.

“And you?” It takes a solid minute for you to figure out his meaning. When you do you smile, shyly, genuinely.

“Oh yes,” you say softly, pressing a kiss into the pad of his thumb.

“Who was your first?”

“A boy named Dane,” you say, recalling the memory, “I was 15. He was nice,” you chuckle to yourself and add, “he wasn’t a very good kisser, but neither was I, I suppose.”

“And now?” he asks. He’s never prodded you this much and you’re reveling in it, leaning deep into his space. He’s dressed down to a shirt and trouser pants, bereft of armor except his helmet. He looks soft like this, pliable. You could sit in his lap and feel him, if you wanted. Instead, you lean back a bit, give him space.

“I’ve had lots of practice,” you say and leave it at that, returning to your own side and lying down, arms folded behind your head. In the moonlight, you’re blushing. Several minutes go by in silence and you’ve started to notice the chill from outside. You’re about to ask Mando if he’d like to go inside when he says:

“I’ve never slept with anyone.” The silence that follows is deafening. You’ve assumed this, but having it confirmed is something else entirely. You struggle for words, any words, to respond to this confession until you realize with abrupt clarity that he doesn’t want that. He just wants to share. You decide you have something you wanted to tell him, too.

“I can’t have children,” you say, almost too soft for him to hear, “but I’ve always wanted them. I always thought I’d… be a good mother.” There’s silence on his end. Then –

“You are a good mother.” It takes about a minute to discern his meaning, but by then you’re blinking back tears.

———————————————————————————————————-

The hatch opens an hour later. The Child’s asleep in his cot, hidden away while you continue your sewing.

“Mando?” you call, peeking around the corner of his cot. “Did you forget something?” He makes his way into your field of vision and you smirk at him, but something in his posture is… off. You realize why moments later.

“Who do we have here?” asks a male voice behind the Mandalorian. From behind his shoulder comes a bald man, dressed in weaponry. And then another man, with red skin and horns, a droid, and the nastiest-looking woman you’ve ever seen. You immediately seek out Mando. He’s looking at you but he refuses to meet your eyes.

“Oh, a little slave girl!” cries the woman with the purple skin, jumping in the air and clapping excitedly. “This will be fun!”

———————————————————————————————————-

“What’s your name?” you ask him once between the breezes of the wind and the phases of the moon. You aren’t expecting an answer, and then -

“Din,” he says breathlessly and quick, as if he’s been waiting for you to ask, “It’s Din.”

“Din,” you say with reverence, caressing the side of his helmet. “I’m so glad to know you.”

———————————————————————————————————-

You should have told him no. Demanded to be sent to Sorgan. That’s the only thought running through your head now, watching him interact with the ghosts of his past. You learn their names after an hour: Mayfield, Burg, Q9-0, but it’s the last one, Xi’an that gives you the most pause. You watch how she circles Mando, strokes her hand down his arm, flirts with him. You know your immediate reaction is jealousy but the more you watch them interact, the more protective you begin to feel. He’s like a livewire around these people, on edge. Mayfield reaches to grab your arm and Mando reacts instantly, catching his wrist with a quiet don’t. But when Xi’an gets close to him… he freezes.

But if you’re interested in Xi’an, she’s twice as fascinated by you. She watches you for hours while you move back and forth, keeping yourself busy. You and Mando shared a look when you’d picked up one of the child’s blankets off the floor, deciding through an unspoken dialogue not to allow these criminals to know of the Child’s existence. All the while she watches you, clicks her tongue. Sometimes she makes remarks that have the hair on the back of your neck standing up.

“What does he have you do for him, huh?” Xi’an asks coquettishly, brushing a lock of your hair behind your ear. “I have a few ideas… but I don’t think Mando shares my appetites. Do you, sweetie?” Mando’s at your side immediately, manhandling you behind him.

“Not even a little bit,” he replies. He steers you away from the rest of the group until you’re as far away as possible, leans close so you can have a semblance of a private conversation. “I’m sorry,” he says, and you know he means for everything.

“I know,” you respond, “this is bad.” You trail off, not wanting to guilt him anymore than you’re sure he already is. There’s something that’s been nagging at you, in the back of your mind. You lean in closer, grabbing ahold of his forearms to stabilize yourself. “Mando, is she… the one you told me about?” All he does is stare, for a bit, and then he nods. Just once. You squeeze his arm and try to stave off your desperate need to hug him. “Okay.”

———————————————————————————————————-

“Have you ever been in love?”

This night, you’ve been out on the hatch for hours, stars long since forgotten, preferring to stare at each other instead. Mando may as well be a star to you, making you burn hot in your core just to look at him. Maker, you think, watching him watch you, will this fire ever stop burning?

“I – uh - no,” he says. “Once, I thought – but she wasn’t a good person.”

“Oh?” You ask, hoping he’ll tell you more. You like to learn about him.

“She was a criminal. She made me forget who I was. Hurt me. I don’t… like who I was when I was with her.” The words seem like an admission. You move the hand that isn’t propping your head up to intertwine your fingers with his. You pull his hand to that it rests over your sternum, stroke your fingertips down his forearm.

“I like who you are now,” you say, hoping this will ease his pain, if only a little bit. The tilt of his head lets you know he’s smiling.

“And you?” he asks, “Have you ever been in love?”

“Oh yes,” you reply, breathless, and whisper for you in your head so many times you hope he can hear you.

———————————————————————————————————-

“She stays,” Mando says sharply. You’re sure he’s close to snapping, fingers digging into the meat of your arm. It’s starting to hurt.

“She comes,” says the Devaronian, arms crossed tight across his chest. “I don’t trust her here alone with Q9.”

“She stays,” Mando repeats, “she’d just be a liability. She doesn’t know how to fire a blaster.” Xi’an quirks the skin above her eye.

“I wonder more and more what the point of her is,” she says, messing with her blaster a bit. “C’mon Mando. Give her a blaster and lets go.”

“Just do what they ask,” you whisper to him, “I’ll stay out of the way. Let’s just get this done so we can get the hell out of here.” He nods once. Then you’re off.

The betrayal doesn’t come as a surprise to you. The murder does. You’ve watched Mando fight before, watched him shoot a bounty but you’ve never watched someone beg for their life before being killed in cold blood. You watched him fight with himself, trying to choose between safety and duty. You wish he’d been more selfish. Your body’s gone numb and you’re dissociating, mind foggy. Mando talks to you but you don’t hear him.

“Hey, hey,” he says, smacking your shoulders, “come back to me. Come back. We have to finish this, okay? I’m sorry, I’m so sorry – you never should have, should have been here. But we need to get out. Okay? Lets finish this.” You nod, following his voice back to your body. Its hard work but you manage, and when you look away you realize you’re locked in a cell.

“Okay, okay,” you say, repeating the word over and over until you feel mostly yourself. “Let’s do this.”

———————————————————————————————————-

“Can you never marry? Settle down? Have a family?”

This is a dangerous game you’re playing, yet you can’t stop. Every star-filled night you’ve been building up to this question, needing to know the answer. There’s tension between the two of you that surpasses sexual. You know he cares for you. You have to know if there’s a possibility for more.

“My clan is… very strict. There aren’t many Mandalorians left. Our lives are to be devoted to the creed.” He doesn’t sound disingenuous, but he does seem… detached.

“Is that what you want from life?” you ask softly, watching him from the corner of your eye. “Life experienced from the inside of a helmet? Forever?”

He stares blankly back at you; no head tilt, no external indication of what his facial expressions might be telling you. You blanch.

“I didn’t mean to offend you,” you say quickly, grabbing for his knee, “I only meant… I mean… Are you happy?” He takes some time to respond, but when he does he takes your breath away.

“It’s the only life I’ve known,” he says, resting his hand atop yours, “but that doesn’t mean it’s the only one. I’m… happiest when I’m with you.”

Your eyes close involuntarily, lips curling into a smile.

Oh Maker, you pray, happy tears stinging your eyes, let me love him.

———————————————————————————————————-

You awake to Mando shaking your shoulder and saying your name gently. You blink your eyes open to find most of the main lights of the ship turned off, the Child nowhere to be found.

“He’s in his cot,” says the Mandalorian to your unspoken question. “Come.” He stands and extends his hand, which you take without hesitation. He leads you to the hatch, open it, and leads you outside. He’s brought you to another barren planet to watch the stars, only this one is… different. You gasp audibly, bringing your hands to cover your mouth. You’re fully awake now, eyes wide open in an attempt to take everything in because.

Because wherever you are it the largest moon you’ve ever seen, enough surface area that, when the sun shines upon its surface, it gives off enough white light that everything on the surface of the planet is clearly visible. But the sky is still inky black, and millions of stars and planets can be seen from the ground. It’s a wonderous juxtaposition, one you didn’t know what possible. You turn to the Mandalorian in awe, hoping for an explanation.

“I tracked a bounty here once,” he says, shrugging his shoulders, “I thought you might like it.”

“I do,” you say, with enough feeling that you might choke on it.

This scene is so similar to the ones before it and yet there’s an air of something. You can’t quite name it, but there’s a change between the two of you. You can feel is in the way he stares at you, how you can practically feel the heat coming off his eyes. His gaze scans your entire figure, ankles to eyes. It makes you flush. You’re both lying on your backs looking at the stars, so mesmerized that you haven’t spoken a word in an hour. You break the silence because he has to know.

“I didn’t like the way she was looking at you,” you say, shifting so that you’re now on your knees. Mando watches you shift and then does the same, sitting up on his haunches.

“Xi’an,” he says. He doesn’t need to ask. You nod.

“I hate to see you look scared. Unsure.” Your hand snakes its way to his in the grass. Once you find it, he allows you to curl your fingers in his.

“I’m okay,” he says, and that’s that.

Except it’s not. He can’t brush this aside, you wont allow it, because there’s a tightening happening in your chest, like a fist around your heart and you know with sudden and shocking clarity that there will never be another. He is it. He has to know.

Slowly, so as not to startle him, you shift your right hand to unclasp your fingers. They dance across his hand, up his arm, over the slope of his shoulder, up his neck. He stiffens but doesn’t push you away, trusts you, and the truth makes your heart ache. You stop once your palm has circled his neck, finger tips able to get a little ways into his helmet. You feel stubble there, scratching your palm.

His breathing has picked up, the sound of it tinny through the modulator. After several moments of deep breaths he mimics your movements, right hand gliding up your body. His palm cups your cheek and he swipes his thumb over your lips in a gesture you’ve become familiar with. You stare at each other like this for what feels like hours, mimes of each other. Then he drops your gaze and looks at the grass, at your legs, anything but your face.

“There are things,” he begins, “that are normal for others. Expected. But not for me.” He’s staring now; waiting for your response, you imagine. You know what he wants you to say. To smile and nod and accept this answer. Leave everything unspoken, as you usually do. But not this time. You’ve been aching for months to put a name to your plight and now that you have one you must share it.

I understand, you think.

“I love you,” you say instead. He jerks so violently away from you that you’re afraid you’ve physically hurt him. He drops your jaw and your palm is remove from his face by the force in which he moves away.

“I… can’t,” his voice is breathless, desperate, scared. All at once you know that it’s him that’s holding him back, his trauma, not his devotion to his culture. He’s afraid to love you. It makes tears spring to your eyes. You catalog everything the Mandalorian has been through; the pain, the hardship. Broken and touch-starved. But he’s so soft and he loves the Child and no matter what he says you know that he loves you.

“I know,” you say, tipping your head forward so your forehead rests against his helmet, hands cupping the side of it like a promise. “I know.”

#did it work???????????#din djarin x reader #din djarin #the mandalorian x reader #din djarin x you #ok folks this is the last time #if it doesn't work it doesn't work #my writing #*

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peakytoms · 6 years

Text

NSFW ABC’s ~ John Shelby

A = Aftercare (What they’re like after sex)

So things with John boy never get too wild so aftercare is not really necessary. He likes to just lay in bed and talk to you about his day while you two share a few cigarettes .

He also loves eating after sex because he expels so much energy during the deed—so he will usually go downstairs and drum up some bread and jam and bring them back upstairs for you and him.

The bed always gets crumby but you don’t care “that’s what the maids for” says JOhn

B = Body part (Their favourite body part of theirs and also their partner’s)

He is obsessed with you butt—he’s a butt guy. He loves backing that ass up against his hips anytime of the day, loves you sitting on his lap when he’s at work or at home in the office. Big time butt guy.

Hes so much of a butt guy, he even likes his own ass. He knows he was sculpted by Michelangelo himself and he has likes to show off what god gave him.

When the two of you are home without the kiddos—the both of you are always naked, beauty should not have to be covered up

C = Cum (Anything to do with cum basically… I’m a disgusting person)

We all know our dear boy john has a massive impregnation kink—he loves knowing his woman is around carrying his seed and making it into a human baby. As such, his favorite thing to do is cum inside of you. He also loves to watch himself spill out of you when its all over so he likes to make sure his woman is filled 😉

D = Dirty Secret (Pretty self explanatory, a dirty secret of theirs)

John is one dirty dirty boy (you’ve seen him smirk right? That smirk is LOADED with dirty filthy secrets)

At dinner with Polly or the rest of the fam—he sneaks one of your hands down to his already hard cock. He thinks hes so suave and that no one notices (but because of that infamous smirk—everyone always knows what is happening but lets him have him moment)

When the two of you were younger, you had sex on Pollys bed (she had a bigger bed so it made sense at the time—it’s a secret you two will keep til your DEATHS)

E = Experience (How experienced are they? Do they know what they’re doing?)

John Shelby mates like a bunny rabbit…plenty of experience has been had

F = Favourite Position (This goes without saying. Will probably include a visual)

Given how many times you have probably been pregnant (John has like 3 million kids? 3 billion? Ive lost count) Johns fave position is anything from behind (plus that way he gets a perfect view of your tush which as discussed he is obsessed with. Plus, taking you from behind is super convenient

G = Goofy (Are they more serious in the moment, or are they humorous, etc.)

He is a goofballTM and sex usually is goofy as well. Because of all the kids you two have had, you cant take time for granted and you often need to be creative because children are literally everywhere and sometimes you don’t even realize it. So sometimes things just get goofy (kids walk in, gotta make quick worl to cover up—and the eventually get them out so yall could finish)

H = Hair (How well groomed are they, does the carpet match the drapes, etc.)

John is practically bare down there. He thinks the hair just covers up all that god given beauty so he doesn’t like to keep it around.

I = Intimacy (How are they during the moment, romantic aspect…)

He tries his best to be romantic. He loves you and all that you have done for him so he wants to do his best to make sure he treats you like a QUEEN TM but ya know…life happens and god bless him for having good intentions and ideas but John is not the best at executing them. He tries to be romantic and sweet, but when the time actually comes around there just isn’t any time for romance (all the kiddos running around remember?). But it’s the thought that counts

J = Jack Off (Masturbation headcanon)

You ALWAYS walk in on him while hes jacking his willy. The man has got a thousand and 3 kids, and you get busy, he gets it—but he has needs and if hes stressed (because idk…Tommy wants him to kill his teacher or something) he just needs to release some tension (in his balls) and get on with his life

If you catch him in the moment though—you usually throw those kiddies out and help out your man

K = Kink (One or more of their kinks)

Getting you pregnant

ou being pregnant (your tits get almost as big as your ass and as discussed our boy john is a man of the tush)

Fucking you while pregnant—he one kinky guy and he likes the idea of filling you with his seed even when you are currently growing his seed into a human child

He likes doing it in public spaces (especially if Polly is around—he likes to think hes outsmarting her even though she knows everything…she is omniscient…omg shes a godTM

L = Location (Favourite places to do the do)

The Office because there are no kids at the office, or in the car on the way to the office because that man just has no patience

M = Motivation (What turns them on, gets them going)

Seeing you pregnant, seeing you be a mama, stress because his brother is a dick, having a really good meal, getting drunk, a really calming cigar, bashing someones head in, blinding someone, winning some money at the races… basically Johnny is always a horndogTM and everything gets him going.

N = NO (Something they wouldn’t do, turn offs)

He’s too needy and desperate at most times to ever be picky with things to try

That being said, you two have tried some rougher sex but it just wasn’t his thing. Even though hes a blinder and a savage man with his enemies, he doesn’t really like hurting people (especially someone he loves a helluva lot)

Also for some strange reason, you getting close to any of his brothers or blinders just rubs him the wrong way and he does not like it (I wonder why…)

O = Oral (Preference in giving or receiving, skill, etc)

Giving

Youre a good mama and are nearly always stressed and tired because of all the billions of his kids running around small heath so Johnny boy likes to take care of his girl and do all the work to show his appreciation

P = Pace (Are they fast and rough? Slow and sensual? etc.)

Because yall never really have that much time, things are usually fast and rough—but not rough rough, just rough because he has like 3 seconds before a kid starts crying and he just gets very vigorous. If the kids are away at Pols, he will tgry his best to make it special—all romantic and slow and sensual and wooowwwwweeeeeee im feeling john rn what is this?

Q = Quickie (Their opinions on quickies rather than proper sex, how often, etc.)

Pretty much the only way he has sex is with quickies because he has like 651360176 children somewhere

R = Risk (Are they game to experiment, do they take risks, etc.)

He is always down to find new and creative ways to get wit chuuu—he needs it because when you have kids you need to get creative and inventive

S = Stamina (How many rounds can they go for, how long do they last…)

Because he usually has so little time, he has trained his body to last as quickly as possible (relax though guys he doesn’t cream in like 0.05 seconds, but it usually takes him less than 7 minutes). BUT he is still a shelby and a frickin horse (not a cavalry horse though mind) so he can go for many many rounds (he just hopes he has the time)

T = Toy (Do they own toys? Do they use them? On a partner or themselves?)

Before the kiddies he liked very much to use toys on you, especially blindfolds (he really liked to play up the name “peaky blinders”)

But now that kids are a part of your reality, being blinded just isn’t practical because one of the 6142782 kids could sneak in and see any second and you must remain vigilant

U = Unfair (how much they like to tease)

Like I said, John has had to get very creative and that includes a lot of teasing. And in turn you tease him right back

V = Volume (How loud they are, what sounds they make)

He is naturally just a very loud and boisterous individual and its hard to contain himself. Given the position you two are usually in during the deed (your back to his chest and he rams you from behind) he usually muffles his moans and groans and screams into the skin of your shoulder or neck.

Wild Card (Get a random headcanon for the character of your choice)

So we know how John is a massive horndogTM and gets especialluy aroused whenever he sees you be a good mama, but you are the same way with him. John boy is a great dad (your fave pet name for him is Mr. Daddy since he likes to pretend to be a big manTM who can be a dommydaddy even though he really cant bless him)

Seeing John be a great father always gets you reared up and ready for another little Shelby baby

X = X-Ray (Let’s see what’s going on in those pants, picture or words)

He thinks he was gods gift to women and there are very visible and anatomical receipts for that belief. He is not only the tallest Shelby brother, but also the biggest

Y = Yearning (How high is their sex drive?)

So incredibly high because he is a horndogTM and you constantly do things that excite him

Z = ZZZ (… how quickly they fall asleep afterwards)

He always stays up just in case you wanna go for another round

#john shelby #john shelby headcanons #peaky blinders #peaky blinders headcanons #omg kat actually wrote something for john shelby and is this a miracle? is this personal growth? what is happening?#yall know Tommy is my boy but writing this made me see john boy in a new light and i dont know how to feel #mine

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lupine-publishers-aoics · 3 years

Text

Lupine Publishers | Experimental Approach, Computational DFT Investigation and a Biological Activity in the Study of an Organic Heterocyclic Compound

https://lupinepublishers.com/chemistry-journal/pdf/AOICS.MS.ID.000177.pdf

Abstract

The title compound TZ1 was synthesized by N-alkylation reaction, and its structure was confirmed by 1H NMR, 13C NMR and IR, it was screened for their in vitro antibacterial activity by the agar well diffusion method against four bacteria, Gram-positive (Bacillus cereus, Staphylococcus aureus) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa). The molecule was studied with the density functional theory (DFT) at B3LYP/6–31G (d,p) level in order to determine the relationship between the molecular structure and the antibacterial inhibition behavior. The molecular geometry, frontier molecular orbitals and Mulliken atomic charge of the compound are investigated to get a better insight of the molecular properties. The molecular electrostatic potential (MEP) for a compound was determined to check their electrophilic or nucleophilic reactivity. The theoretical parameters offer significant assistance to understand the antibacterial inhibition mechanism indicated by the molecule and are in full agreement with the experimental results.

Keywords:5-Chlorosatin derivatives; N-alkylation reaction; antibacterial activity; DFT; Molecular electrostatic potential

Introduction

The 5-Chloroisatin is well documented as an important heterocyclic compound in the field of medicinal chemistry. My recently published book and review [1,2] contain a special chapter, dedicated to the chemistry of 5-Chloroisatin and their derivatives.

The 5-chloro-1H-indole-2 3-dione structure is a heterocyclic compound which easily participates in chemical reactions. Its bonding sites are analogous to pyrrole. As shown in Scheme 1, 5-Chloroisatin is reactive at four different positions including the carbon atom 3, nitrogen atom 1, the C2–C3 p-bond and the C2–N sigma bond.

This moiety of 5-Chloroisatin and their derivatives possess pharmacological and chemotherapeutic properties such as anti- cancer [3], anti-diabetic [4], anti-inflammatory [5], anti-malarial [6], anti-bacterial [7], anti-fungal [8], anti-viral [9] and others drugs for treatment of several diseases [10].

Density functional theory (DFT) has become a convenient method to decipher experimental results, in antibacterial activity; this technique makes it possible to accurately predict the inhibition efficiency of organic compounds on the basis of electronic and molecular properties as well as reactivity indexes [11]. The objective of current study is to explore relationship amongst structure and electronic properties of the synthesized 5-chloro-1- (2- (dimethylamino) ethyl) indoline-2,3-dione (TZ1) using DFT. Then, the evaluation of its antibacterial activity. (Scheme1)

Experimental Details

Chemistry

Melting points were determined using the kofler bench apparatus and uncorrected. The spectra of 1H NMR spectra were recorded in CDCl3 on the Brucker Avance-300 spectrometer, operating at 300MHz and at 75MHz for 13C-NMR using TMS as an internal standard and Spin resonances are reported as chemical shifts (d) in parts per million (ppm). Infrared Spectra were run on AVATAR 320 AEK0200713 spectrometer and frequencies are reported in cm-1. The purity of the synthesized compound was confirmed by thin layer chromatography (TLC), performed on Silica gel 60 coated plates. UV light was used for the visualization of TLC spots [12].

General procedure

The 5-Chloroisatin derivatives TZ1 was prepared by mixing 0.2g (1.1 mmol) of 5-chloro-1H-indole-2,3-dione, (0.23g, 1.16mmol) of potassium carbonate in 15mL of N-N dimethylformamide (DMF) and (0.035g, 0.10mmol) of BTBA, then, the reagent is slowly added, the mixture is left at room temperature for 48 hours. The reaction mixture was concentrated by using rota vapor. The solid was separated out by filtration. It was carefully checked by thin layer chromatography. The compound was isolated by column chromatography by using different fractions of n-hexane and ethyl acetate [13-16].

Yield=89%; mp: 114 °C ; 1H NMR (CDCl3) δppm 7.53-7.54 (m, H, HAr); 7.51 (d, H, HAr, J=9Hz); 6.90 (d, H, HAr, J=9Hz); 3.85 (t, 2H, CH2, J=9Hz); 3.75 (t, 2H, CH2, J=9Hz); 2.15 (m, 6H, CH3). 13C NMR (CDCl3) δppm: 184.59 (C=O); 164.45 (N-C=O); 146.22, 141.13, 110.39 (Cq); 138.59, 126.08, 113.36 (CHAr); 55.90, 46.79 (CH2); 45.09 (CH3). Infra Red (KBr) cm-1: 3565, 3174, 30815 (C-H), 2975, 1720 (C=O), 1607 (NC=O), 1445, 1472 (C=C) 1185,1123 (N-C), 654 (C-Cl).

Antibacterial screening

Synthesized compound TZ1 was screened for their antibacterial activity against two Gram positive (Bacillus cereus and Staphylococcus aureus) and two Gram negative (Escherichia coli and Pseudomonas aeruginosa) bacteria by the agar well diffusion method, using LB medium (Luria Bertani medium: yeast extract 5.0g, peptone 10.0g, sodium chloride 5.0g, distilled water 1000mL). This technique was recommended by CLSI [17].

A sterile paper disk was placed on the surface of each plate and impregnated with 5μL of the TZ1 solution at a final concentration of 10mg/mL. Then, the plates were incubated at 4 °C for 2 hours to permit good diffusion before incubation at 37±2 °C for 24 hours. The diameters of the inhibition zones were measured in mm with the caliper. A disc impregnated with 2% dimethylsulfoxide as a negative control was made the experiment was carried out in triplicate.

In order to determine the Minimum inhibitory concentration (MIC) values, we started by the dilution of the TZ1 was prepared in a Mueller Hinton broth supplemented with bacteriological agar, to reach a final concentration between 5mg/mL and 0.004mg/ mL, 50μL of bacterial inoculum was added to each well at a final concentration of 106CFU/mL. DMSO (2%) was used as a negative control. The final concentration of our product was between 5mg mL-1 (3rd well) and 0.019mg mL-1 (well 11). Plates were incubated at 37 °C for 24 hours. After 2 hours of a subsequent incubation, bacterial growth was revealed by reduction of blue dye resazurin to pink resorufin [18].

Including, the minimum bactericidal concentrations (MBC) which is the last step in the protocol, a bactericidal control is carried out 24 hours earlier by streaking on a platelet agar, after microdilution to the broth by spreading 5μL of the negative wells on Luria Bertani agar plates.

Theoretical calculations

The computational studies of compound TZ1 were performed using the GAUSSIAN 09W [19] program package and visualized with the Gauss View on a personal computer using density functional theory (DFT) method with 6−31G (d,p) as the basis set [20]. The using HOMO and LUMO orbital energies, the ionization energy and electron affinity can be expressed as: IP = -EHOMO, EA = -ELUMO, respectively. The total hardness, η and electronegativity χ were given by the following relations: [21].

Result and Discussion

Synthesis of 5-chloro-1-(2-(dimethylamino) ethyl) indoline- 2,3-dione (TZ1) is outlined in Scheme 2, it was prepared according to a similar previous procedure [22]. 5-chloroisatin was used as a starting material for the synthesis of various substituted indole derivatives [23-27].

The (TZ1) was synthesized by the N-alkylation reaction of 5-chloro-1H-indole-2,3-dione in DMF, a base K2CO3 and a TBAB catalyst was added to a stirred solution at room temperature, Chloro-N,N-dimethylethanamine was added dropwise to the mixture under conditions of catalysis by phase transfer for 48 hours, the reaction was monitored by thin layer chromatography. After this time, the mixture was filtered and concentrated and dried under vacuum to afford the required product. The complex was obtained in good yield, stable in air, and is colored solid, soluble in methanol, chloroform, DMF, and DMSO.

The 1H-NMR, 13C-NMR and IR were used to assign the structure of synthesized compound. (Scheme 2)

Antibacterial activity

The In vitro antimicrobial screening tests of synthesized compound TZ1 was carried out as an antibacterial activity. the tested compound showed biological activity against different types of Gram-positive (Bacillus cereus and Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli), it showed zones of inhibition of MIC/MBC values ranging from 0.156/0.156 to 0.313/0.313mm against the Gram-positive bacteria and between 0.625/0.625 and 1.25/1.25mm against Gram-negative bacteria.

Coordination enhances the antibacterial activity and the TZ1 in the present study are more active against Gram-positive than Gram-negative bacteria [28]. On the other hand, it should also be noted that the presence of nitrogen and oxygen atoms which are the highest values of the negative charge on the molecule TZ1 suggesting that these centers have the maximum electron density and would preferentially interact with the micro-organisms Gram positive then increases the antibacterial potential.

Computational details

Frontier orbital energy analysis and other global reactivity descriptors: The all optimized structures along with the numbering scheme of TZ1 at DFT/B3LYP level using the 6-31+G (d,p) basis are shown in Figures 1-3.

The HOMO-LUMO orbitals help to characterize the chemical reactivity and kinetic stability of the molecule.

The analysis of the HOMO highlights the areas of the molecule that can donate electrons to electrophilic species while the analysis of the LUMO predicts the regions of the molecule with high affinity to accept electrons from nucleophilic species. The calculated HOMO–LUMO energy gap value is found to be 3.1673 eV.

The dipole moment (μ(debye= 5.6982) tells about the polarity of the molecule. The higher value of dipole moment in case of TZ1 molecule is mainly attributed to an overall imbalance in charge from one side of a molecule to the other side is also evident from the MESP plot. DFT calculation gives an idea about the substance reactivity and site selectivity of the frameworks. By the computed value of HOMO and LUMO energy values for the TZ1, the electronegativity (χ), total hardness (η), Softness (σ), can be calculated. The significance of (η) and (σ) is to evaluate both the reactivity and stability [31].

Molecular electrostatic potential (esp) map: The molecular electrostatic potential mapped surfaces show the charge distributions of molecules three dimensionally which give clear and special signature of the interactions of the compounds [31].

The molecular electrostatic potential is related to the electronic density and a very useful descriptor for determining sites for electrophilic attack and nucleophilic reactions as well as hydrogenbonding interactions [32].

The MEP mapped surface of the compound TZ1 was calculated by DFT/B3LYP at 6 31G (d,p) basis set and MEP surface are plotted in Figure 6. Red, blue and green colors represent regions of the most electro negative, most electro positive electrostatic and zero potential, respectively [33].

Mulliken charges analysis

The Mulliken atomic charges have a significant role in the application of quantum chemical calculations to molecular systems, by determining the electron population of each atom as defined by the basis function [34]. Table 3 exhibits the calculated mulliken atomic charges except for atoms H by DFT/B3LYP at 6 31G (d,p) basis set. Also, the color range in the scale of positive and negative charge and graphical representation for Mulliken atomic charges of TZ1 is shown in Figure 7.

From the listed tabulated values (Table 3) of atomic charge, we can summary that the charge on the carbon atom (C6) is greater than other carbon atoms in the all compounds because it is connected to electronegative chloride atom (Cl10). Then, all nitrogen and oxygen atoms (N13, N22, O14 and O15) are the most negatively charged ones, suggesting that these centers have the maximum electron density, which can interact with the positively charged part of the receptor easily.

Conclusion

In summary, we report the synthesis and characterization of 5-chloro-1- (2- (dimethylamino) ethyl) indoline-2,3-dione (TZ1) in excellent yield. The antibacterial activity of TZ1 has been explored experimentally and by quantum calculations. The frontier orbital energy analysis, mulliken atomic charges and electrostatic potential were also studied by using the DFT method at B3LYP/6–31G (d,p). The antibacterial bioassay showed that it possessed excellent activity.

Acknowledgements

The author would like to thank all the people who helped to carry out this work such as 1H NMR, 13C NMR, IR, and for antibacterial activity.

https://lupinepublishers.com/chemistry-journal/pdf/AOICS.MS.ID.000177.pdf

https://lupinepublishers.com/chemistry-journal/fulltext/experimental-approach-computational-dft-investigation-and-a-biological-activity-in-the-study-of-an-organic-heterocyclic-compound.ID.000177.php

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omc-juniperpublishers · 3 years

Text

A Phase I Drug Discovery and One-, Two-, and Three-Class of Indoline-2,3-dione Testing for Anticancer and Cytotoxic Activity-JuniperPublishers

Journal of Chemistry-JuniperPublishers

Abstract

Cancer diagnosis begins with a thorough physical examination and a complete medical history. Laboratory studies of blood, urine, and stool can detect abnormalities that may indicate cancer. Normal ranges of Indoline-2,3-dione scaffolds have been studied. We report here one, two, three novels anticancer target compounds 5-Flouro-1-benzyl isatin -2, 3-b-quinoxaline and 5 -Methyl-1-benzyl isatin -2,3-b- quinoxaline and display good quality of molecular arrangement towards cancerous cells.

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Introduction

Cancer has a variety of forms this definition still holds secrete even today and selection removes variation. Cancer comes homologous proteins can differ in their specificities, specific activities, level of expression or some other basic property. However homologous will have some residual similarity in their form and function. Cancer is a biological event has brought some useful innovations in practice to the study of treatment. It was felt there should not be separate drug discovery standard for reduce the dose frequency and reduce adverse effect and shorten time treatment. The natural history of drug discovery could illustrate the complexity in selecting a baseline. Cancer drug discovery is a relatively slow growing chemical agent with an incubation period said to range from a few year to a life time. If any other anatomical symptoms will see during the laboratory trial, it must be treated thoroughly Indoline-2,3-dione scaffolds is multifunctional expressed by drug discovery series of novel 97 compounds were synthesized for anticancer and cytotoxic activity against different 60 cell line cultures [1]. 07 compounds were selected on molecular recognition and screened for anticancer study [2]. Among those is screened compounds RH8, RH 42, RH84, RH89, RH90, RH 94, RH97 found good quality of anticancer activity against most of the cancer cell line with ranges from -77.23 to 55.85% growth. Target compound 5-Flouro-1-benzyl isatin -2, 3-b-quinoxaline (RH-89) display -77.23 to 7.31% growth & another target compound RH 94 display -64.78 to 65.14% growth against most of the leukemic cell lines, while RH-08 has shown very good activity against CCRF-CEM cell line with -31.85% growths and weak anticancer activity against breast cancer cell line with 53.47% growth, but failed to respond against other leukemic cell lines. RH-89 has also shown moderate activity against nonsmall cell-lung cancer (HOP-62) with 34.69% growth. RH-90 has shown good anticancer activity against leukemic cell line SR with 18.70% growth and weak activity against Non small cell lung cancer HOP-92 and NCL-H322M with 57.14 and 57.20% growth respectively. The cytotoxic property [3] of Indoline-2,3-dione scaffolds, among 3-benzohydrazide (RH 06-64) series, RH-22 and 28 were found to be most cytotoxic against L-1210 cell line (9 and 6μg/ml). RH-33 was found to be cytotoxic against CEM/0 cell line (6.9μg/ml). Among 3-isonicotinohydrazide derivatives (RH 65-72), RH-70 was found to have better cytotoxic property against all three types of cell lines (6.1-6.7μg/ml). From 3-quinazolin-4(3H)-one series (RH 74-82), RH-75 was found to be better cytotoxic against Molt4/8 cell line (6.0μg/ml) and RH-77 was found to have better cytotoxicity against L1210 cell line (9μg/ml) remaining compounds RH-79, 80, 82 have shown moderate to weak cytotoxic property. Among 6H-indolo [2,3-b] quinoxaline derivatives (RH 83-96), RH-92 have shown better cytotoxic property against L1210 cell line (7.2μg/ml).

Compounds and Reagents

All chemicals used were procured from Sigma-Aldrich. The purity of chemicals was checked before use and purified. Melting points were recorded by open capillary tube method and uncorrected. Purity of all the synthesized compounds was assessed by thin layer chromatography on silica Gel-G'(100-200 mesh) plates. Structures of compounds were confirmed by FTIR, 1HNMR and Mass spectra. IR spectra were recorded in KBr disk on Nicolate IR-Instrument and reported in cm-1. Mass spectra were recorded on Shimadzu-LCMS 2010 EV-single quadrapole instrument. 1HNMR spectra were recorded on the BRUKER-300 instrument with TMS as the internal standard in CDCl3/DMSO as a solvent. Total 97 derivatives of Indoline-2,3-dione scaffolds have been synthesized by Sandmeyer process methodology using N-methyl morpholine, substituted benzylchlorides, isoniazid, substituted benzoylhydrazide, quinazoline, orthophenylenediamine, halogens and alkyl groups as substituent and compounds were recrystallized by using ethanol-chloroform mixture. Synthesized compounds and melphalan as a standard were also evaluated for their in vitro cytostatic activity against human Molt 4/C8 and CEM T-lymphocytes as well as murine leukemia L1210 and anticancer activity against Leukemia, Non-Small Cell-Lung Cancer, Colon Cancer, and Breast Cancer. Synthesized compounds were screened for anticancer activity at National Cancer Institute, Maryland, USA and Rega Institute, Belgium respectively (Figure 1).

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Result and Discussion (Tables 1 &2).

Chemistry

RH8; MF C15H10N3O2Cl, Yellowish fluffy mass, MW 299.71, MP 280-82,% Yield 49, IR 3226 (-NH), 3044 (-CH, ar.), 1701 (>CO), 1678 (>CO). NMR 14.03 (d, 1H, NH.), 11.28 (s, 1H, NH), 7.88-7.84 (m 2H, ar.), 7.59-7.42 (m, 4H, ar.), 7.25-7.23 (m, 1H, ar.), 6.87-6.84 (m, 1H, ar.). m/s 299 (299.29)

RH 42; MF C22H16N3O2C., Yellowish-fibrous mass, MW 389.83,'22 16 3O Cl MP 170-72, % Yield 81, IR 3363 (-NH), 3196 (-CH, ar.), 3028 (CH, alip.), 1750 (>CO), 1682 (>CO). NMR 14.04 (s, 1H, NH.), 7.97(d, 2H, ar.), 7.82 (s, 1H, ar.), 7.46 (t, 2H, ar.), 7.48 (t, 1H, ar.), 7.257.04 (m, 5H, ar.), 6.76 (d, 1H, ar.), 4.87 (s, 2H, CH2), 2.25 (s, 3H, CH3). m/s 389 (389.42)

RH84: MF C21H14FN3, Reddish Fluffy Mass, MW 327.35,MP 162-13,% Yield 72,IR 3045-3013 (-CH, ar.), 2954-2849 (-CH,

alip.), 1610, (>C=C), 1580 (>C=N).,NMR 8.51 (d, 1H, ar.), 8.348.32 (m, 1H, ar.), 8.16 (d, 1H, ar.), 7.80-7.76 (m, 1H, ar.), 7.737.69 (m, 1H, ar.), 7.63 (t, 1H, ar.), 7.41-7.31(m, 4H, ar.), 7.00 (t, 2H, ar.), 5.69 (s, 2H, -CH2).,

RH89, MF C21H14FN3, Orange Mass, MW 327.35,MP 220-22,% Yield 68, IR 30(56-3004 (CH, ar.), 2904-2849 (CH, alip.), 1618 (>C=C), 1582 (>C=N)., NMR 8.38 (d, 1H, ar.), 8.24-8.19 (m, 2H, ar.), 7.86-7.82 (m, 1H, ar.), 7.79-7.74 (m, 1H, ar.), 7.42 (d, 1H, ar.), 7.39-7.32 (m, 2H, ar.), 7.31-7.28 (m, 4H, ar.), 5.77 (s, 2H, -CH2).

RH90; MF C22H16FN3, Yellowish Mass, MW 341.38,MP 17722 16 380,% Yield 71,IR 3054-3029 (CH, ar.), 2917-2849 (-CH, alip.), 1613 (>C=C), 1585 (>C=N). NMR 8.33 (d, 1H, ar.), 8.18-8.15 (m, 1H, ar.), 7.81-7.77 (m, 1H, ar.), 7.73-7.69 (m,1H, ar.), 7.37-7.29 (m, 3H, ar.), 7.22 (d, 2H, ar.), 7.12 (d, 2H, ar.), 5.68 (s, 2H, -CH2), 2.30 (s, 3H, -CH3).

RH 94; MF C22H17N3, Reddish Fluffy Mass, MW 323.39, MP 215-18, % Yield 75, IR 3059-3024 (CH, ar.), 2966-2850 (-CHalip.), 1616 (>C=C), 1578 (>C=N).NMR 8.38-8.36 (m, 2H, ar.), 8.20-8.18 (m, 1H, ar.), 7.83-7.79 (m, 1H, ar.), 7.76-7.72 (m, 1H, ar.), 7.49 (d, 1H, ar.), 7.38-7.29 (m, 6H, ar.), 5.67(s, 2H, -CH2), 2.59 (s, 3H, -CH3).

RH97: MF C21H15FN3, Yellowish Mass, MW 309.36,MP 173-75,% Yield 6(3 IR 3048-3030 (CH, ar.), 2908-2856 (-CH, alip.),1610 (>C=C), 1562(>C=N).NMR 8.32 (d, 2H, ar.), 8.14 (d, 1H, ar.) 7.79-7.75 (m, 1H, ar.), 7.71-7.68 (m, 1H, ar.), 7.45 (d, 1H, ar.), 7.32-7.23 (m, 3H, ar.) 6.98-6.95 (m, 2H, ar.), 5.66, (s, 2H, -CH2), 2.55 (s, 3H, CH3).

RH-22; MF C15H9BrFN3O2, Orange gritty powder, MW 362.15, MP >310, % Yield 57, IR 3229 (-NH), 3056 (-CH, ar.), 1681 (>CO), 1620 (>C=C), 1552 (C=N), 1463 (C-H). NMR

RH28; Yellowish fluffy mass, MW 423.06, MP >310, % Yield 84,IR 3263 (-NH), 3035 (-CH, ar.), 2783 (-CH, alip), 1672 (>CO), 1665 (>C=C), 1529 (C=N), 1467 (C-H).

RH65;MF C21H8N4O2Cl, Yellowish fluffy mass, MW 390.82, MP 197-99, % Yield 76 IR3252 (-NH), 3073 (-CH, ar.), 2918, (CH, alip.), 2850 (CH, alip.) 1691 (>CO). 13.87 (s, 1H, -NHCO), 8.873 (d, 2H, ar.) 7.813 (d, 2H, ar.), 7.765 (s, 1H, ar.), 7.611 (d, 1H, ar.), 7.339 (m, 4H, ar.), 7.05 (d, 1H, ar.), 5.018 (s, 2H,-CH2)

RH 72; MF C14H2N4O2ClF, Orange gritty powder, MW 318.69, MP299-300, % Yield 70, IR 3249 (-NH), 3070 (-CH, ar.), 29312849 (-CH, alip.), 1700-1691 (>CO), 1613 (>C=C), 1520 (C=N), 1472 (C H). NMR13.95 (s, 1H, - NHCO), 8.86 (d, 2H, ar.), 7.80 (d, 2H, ar.), 7.5 (s, 1H, ar.), 7.305 (m, 6H, ar.), 6.9685 (d, 1H, ar.), 4.987 (s, 2H, -CH2 -), 2.296 (s, 3H, -CH3).

*50% inhibitory concentration (IC50) value, expressed in uM

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Conclusion

A comparison of the synthesized and evaluated novel Indoline-2,3-dione scaffolds and its series which showed nanomolar activity against most of the leukemia cell line and other cancers cell line. Taste compounds RH8, RH 42, RH84, RH89, RH90, RH 94, and RH97 possess inhibitory activity. Target compound RH89 and RH 94 displays -77.23 to 7.31% and another target compound - 64.78% to 65.14% growth respectively. All the 97 compounds designed analogs with chemical substitutions at the various position confirmed by analytical model showing better interactive scores respectively Molecular recognition studies in the series removal of alky group from 4'-position of N-benzyl ring causes loss in activity (RH-71) and replacement of benzyl group by -H makes the compound inactive (RH-72), while RH-08 has shown very good activity against CCRF-CEM cell line with -31.85% growths and weak anticancer activity against breast cancer cell line with 53.47% growth, but failed to respond against other leukemic cell lines. RH-89 has also shown moderate activity against non-small cell- lung cancer (HOP-62) with 34.69% growth. RH-90 has shown good anticancer activity against leukemic cell line SR with 18.70% growth and weak activity against Non small cell lung cancer HOP-92 and NCL-H322M with 57.14 and 57.20% growth respectively. The comparison of activity with structure similarity and differences in substitution reveals that compounds with quinoxaline moiety are more active than benzohydrazide moiety (RH-89 & 94> RH-08). Further, 5-fluoro or 5-methyl substituted compounds (RH-89 & 94) were more active than un-substituted analogues (RH-97). It was also observed that any substitution on the 4' position of N-benzyl ring causes loss in activity (RH-90 & 97). Similarly among benzohydrazide series compounds with chlorine substitution at 5th position is active (RH-08), however N-benzylation reduces the activity (RH-42). However role of N-benzyl group has mixed effect on the activity. The cytotoxic results of benzohydrazide series show that RH-22 and RH-28 were most cytotoxic against L-1210 cell line (9 and 6μg/ml). RH- 33 was found to be cytotoxic against CEM/0 cell line (6.9μg/ml). The activity report shows that presence of halogen increases the cytotoxic property. Replacement of halogen from 4' position of benzohydrazide causes loss in activity as in case of RH-06, 25, 26, 28 and 31. Among 3-isonicotinohydrazide derivatives RH-70 was found to have better cytotoxic property against all three types of cell lines (6.1-6.7μg/ml). Removal of alky group from 4'-position of N-benzyl ring causes loss in activity (RH-71) and replacement of benzyl group by -H makes the compound inactive (RH-72). It shows that halogen at 5th position and alkyl group at 4' position of N-benzyl ring is essential for activity. From 3-quinazolin- 4(3H)-one series RH-75 was found to be better cytotoxic against Molt4/8 cell line (6.0μg/ml) and RH-77 was found to have better cytotoxicity against L1210 cell line (9μg/ml). Among 6H-indolo [2,3-b]quinoxaline derivatives RH-92 has shown better cytotoxic property against L1210 cell line (7.2 μg/ml) which shows the importance of halogen at 5th position and presence of N-benzyl moiety in the compound. Alteration in the combination leads to loss in activity (RH-84, 87, 88, 89, 90, 91, 93-96). Based on the results without any halogen has either very weak activity or inactive and none of them are close to the standard Malphalan (2.1-3.2μg/ml) [4-11].

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Acknowledgment

This study has been guided under the guidance of Renowned Laboratory Scientist Respected Dr. Ramesh Paranjape, Founder National AIDS Research Institute India. I express my sincere gratitude towards Respected Sir for motivation and being great knowledge source for this work.

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juniperpublishers-ttsr · 4 years

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Olive (Olea europaea L.) of Jerusalem in Palestine

Abstract

The olive (Olea europaea L., Oleaceae) is the most important fruit tree found before more than 6000 years, which contributes to the increase of the agricultural national product in Palestine, and therefore plays a role in increasing the Palestinian economy. Methodology, we analyzed data on environmental factors of Jerusalem (East Jerusalem), including climate and bioclimate, using the bioclimate classification for the earth of Salvador Rivas Martinez during study period 1993-2012, which we obtained from the Palestinian Meteorological Station and the same period of olive production (rainfed) from the Palestinian Central Bureau of Statistics. Result and discussion, when we used a correspondence analysis (CA), observed that Jerusalem affected negatively by bioclimate factors as annual ombrothermicindex and simple continentality index during the years (1993-1997), whilst, the effect was positive for years (1997-2012), with a large proportion of the variance explained by axis F1 (96.81%), axis F2 (2.88%) and axes F1and F2 (99.100%). Furthermore, in Jerusalem, the optimum of mean monthly temperature for increased olive production is between 15-200oC, with the result of annual ombrothermic index value more than 3.5, compensated thermicity index (210/210 to 350/350), simple continentality index between (15-20). Addition to olive production favor, where the environmental areas thermomediterranean to mesomediterranean of thermotype and dry to humid of ombrotype, and Jerusalem is belonging to mesomediterranean of thermotype and dry to sub humid of ombrotype.

Introduction

Posture problems in youth is rising right under your nose. Are you a parent reading this article? Do you suffer from neck or back pain after a long day at work? Headaches maybe? Did you wake up with pain? Maybe you didn’t, but the other 65% of working population did, dose and do go thru their days in discomfort. You don’t stop eating because you are afraid of cavities? Then why do you expect the millennial to give up their smart devices? How many children do you see already in poor state of health? How many do you know personally? Just like cavities, using smart devices puts us at risk. It’s not about “What is wrong?” it’s about how are were reacting to it individually? What is the compelling reason to make a change before becoming the victim of the consequences?

Methodology

Study area

Jerusalem is a Palestinian city built by the Canaanite Arabs more than 6,000 years ago, occupied more than 18 times throughout history, and the last time by Israel in two stages, the first phase in 1948, was called West Jerusalem, and the second in 1967 (Six-Day War), was called East Jerusalem (people Palestinian area). Finally came the promise of the unjust US President Donald Trump to devote the occupation of the city and considered it the capital of Israel and not the right of the Palestinians, but in fact Jerusalem is the capital of occupied Palestine and one of the holiest cities in the world, the United Nations and the international community do not recognize Jerusalem as the capital of Israel. East Jerusalem is considered part of the Palestinian territories and is not recognized as a Jewish state [25], it is 760 meters above sea level and 64km2 , with coordinates 31.76°N 35.25°E, the city of Jerusalem is located in the center of Palestine, which is about 1km away from the Mediterranean, about 11 km from the Dead Sea, bordered on the eastby the city of Jericho (Jordan Valley, Jordan River and Dead Sea), and on the west by the towns of Ramle and Lod (Meditrranean Sea), on the north by Ramallah and from the south Bethlehem and Hebron cities (Figure 1).

data

In this paper, both climatic and bioclimatic factors were analyzed, data were obtained from the Palestinian meteorological station (Figure 2), with analysis of the olive production data for the city of Jerusalem obtained by the Palestinian Central Bureau of Statistics (PCBS) and for the same years of study for both environment factors and plant production (1993-2012) (Table 1).The analysis of climate and bioclimate factors was done using the Salvador Rivas Martinez to classification of the earth [26-30], and climate factors were consisted of mean monthly temperature (Tm), precipitation (P or ppt) and soil water reserve (R); and bioclimatic factors or variables as compensated thermicity index (It/ Itc), annual ombrothermic index (Io), simple continentality index (Ic) and water deficit (Wdf or Df). Annual ombrothermic index, Io = Pp/ Tp, where is Pp = positive precipitation and Tp = positive temperature (in this case equivalent to annual precipitation and mean annual temperature divided by 12, as all the months have an average temperature above 0; bimonthly summer ombrothermic index, Is2 = P July + August/T July + August; trimonthly summer ombrothermic index, Is3 = P June + July + August/T June + July + August; and simple continentality index (Ic) = Tmax-Tmin, Tmax = maximum temperature of the averages of the warmest month of the year, and Tmin = minimum temperature of the averages of the coldest month of the year; thermicity index, where applicable compensated thermicity index, It/Itc = (T + M + m)*10, where is T = average temperature of the months; T = mean monthly temperature; M = average of the maximum temperature of the coldest month of the year, and m= average of the minimum temperature of the coldest month of the year, and P = precipitation of the months indicated.

Yield: Kilogram/dunum; T or Tm: Mean Annual Temperature; P or ppt: Precipitation; R: Soil Water Reserve; Df or Wdf: Deficit water; Io: Annual Ombrothermic Index; Ic: Simple Continentality Index; and It/Itc: Compensated Thermicity Index; and Olive Production.

Exploratory data analysis

Further, we also used normality tests for Jarque-Bera and Shapiro-Wilk to conduct the analysis [31-34], and we applied an analysis of variance (ANOVA) tests analysis to each of the eight environmental variables as climate and bioclimate factors and olive production, four bioclimatic parameters and the three climate variables or climate factors to obtain the principal component analysis, factor loading, adjusted r-squared, standardized coefficient and regression coefficient (R2) to determine the impact of environmental variables on plant yield. Addition to, exploratory data analysis was used to determine the most related environmental variables for the olive, and the XLSTAT software program were used done. Furthermore, we can use the adjusted r-squared formula is:

where: N is the number of points in your data sample, and K is the number of independent repressors; and linear regression is a way to illustrate the relationship between two variables. The equation has the form Y= a + b X, where Y is the dependent variable, X is the independent variable, b is the slope of the line and a is the y-intercept.

A regression coefficient is the same as the slope of the linear regression equation, and the formula of the regression coefficient that is: B1 = b1 = Σ [ (xi – x) (yi – y)] / Σ [ (xi – x)2]. “y” in this equation is the mean of y and “x” is the mean of x.

Yield: Kilogram/dunum; T or Tm: Mean annual temperature; P or ppt: precipitation; R: Soil water reserve; Df or Wdf: Deficit water; Io: Annual ombrothermic index; Ic: Simple continentality index; and It/Itc: Compensated thermicity index; and olive production.

Result and Discussion

Impact of climate & bioclimate factors on olive production

Principal component analysis (PCA):After we analyzed the climate and climate factors using the Salvador Rives Martinez methodology [29,30], and applied the natural tests of Jarque - Bera and Shapiro-Wilk [31-34], and finally the XLASTAT Software program, the probability value obtained from the studied variables tends to be less than 0.05, which is traditionally acceptable. PCA is used as a in exploratory data analysis, factor loading, standard deviation, covariance, eigenvalues, eigenvectors and other values.

Eigenvectors and eigenvalue:In linear algebra, eigenvector of a linear transformation is a non-zero vector that changed only by a standard factor when that linear transformation is applied. Eigenvalues and eigenvectors using either the methods of matrices or the linear transformations [35,36]. Further, calculate of the eigenvectors and eigenvalue given by: Let M be an n × n matrix. A is there is a nontrivial solution x of Ax = λx. Where, V is an eigenvector of M, if M (v) = λv, λ is called the eigenvalue associated with v. For any eigenvectors v of M and scalar a, M × av = λav. Thus, we can always choose eigenvectors of length 1:

If M has any eigenvectors, it has n of them, and they are orthogonal to one another, thus eigenvectors can be used as a new basis for an n-dimensional vector space, and AX = λX, for some scalarscalar λ. The scalar λ is called an eigenvalue of A, and x is called the eigenvector of A corresponding to the eigenvalue λ.

Factor loading analysis:However, principal component analysis was used to help determine various variables, using the extract factor with eigenvalue > 1 after varimax rotation. The results of PCA, including eigenvalues with a varimax rotation and the factor loadings, are classified in (Table 2) and (Figure 3), there are three of the eigenvalues were found to be >1 (Figure 3). While the total variance for these factors are about (99.60%), and two of the eigenvalues were found to be ˃ 1 and the total variance for the two factors (F1 and F 2) are about (99.60%), as in the (Figure 4). Otherwise, we applied the coefficient of correlation between all environmental variables and factors, and the correlation coefficient is bound to the range of ± 1, the +1 value inform us that the points (xi, yi) determine a straight line with a positive slope, a value of -1 inform us that it specify a straight line with a negative inclination, while the value 0 indicates that there is no correlation of y on x or vice versa.

The factor 1 was dominated positively by the factors of precipitation (0.04), simple continentality index (0.06), and annual omrothermic index (0.09), except water deficit, compensated thermicity index, and mean monthly temperature are dominated negatively (-0.03, -0.07 and -0.03) and accounts for 96.8% of the total variance and cumulative (Figure 4). Factor 2 is a high dominated by mean monthly temperature (0.002), soil water reserve and deficit water (0.001), compensated thermicity index (0.015), while precipitation (-0.03), simple continentality index (-0.01), and annual omrothermic index (-0.03) were dominated negatively. Factor 3 is a dominated positively by soil water reserve (0.008), but with negatively impact for the rest of factors, as reported in the (Table 2).On the other hand, when we applied a correspondence analysis (CA), observed that Jerusalem affected negatively by annual ombrothermicindex and simple continentality index as the bioclimate factors and precipitation as climate factors during the years (1993-1997) because its lies in the negative y-axis (Figure 4), whereas, the effect was positive during the years of study from (1997-2012), with a large proportion of the variance explained by axis F1 (96.81%), while axis F2 (2.88%) and symmetric plot axes F1and F2 (99.60%), this is evident in (Figure 4). Otherwise, the analysis of variance (ANOVA), with a 95% confidence interval has been applied to olive yield, with the seven environmental variables (Tm or T, P or ppt, R, Df or Wdf, Io, Ic and It/Itc), explain significant differences in olive production, impact of these factors on the olive productivity. The results of the analysis for each of regression coefficient, standardized coefficient, and adjusted R² showed that there was a difference significance in this study, in case of olive productivity shows a better regression coefficient with value of mean monthly temperature and compensated thermicity index (R2 = 0.975 and 0.912), adjusted R² (R2 = 0.962 and 0.868) respectively, being closed to 1 (Figure 5 & 6), and negatively with the standard coefficient (-0. 978 and -0.955), because the histograms were negative (Figure 7 & 8); precipitation is a better linear regression or regression coefficient and adjusted R² with the values of (R2 = 0.968, 0. 952) respectively, being closed to 1 (Figure 9), and correlated positively with the standard coefficient (0.984), because the histograms is positive (Figure 10), and the rest of variables and regressions values as tabulated in the (Table 3).

Olive in jerusalem

Jerusalem known by other names in Arabic such as: Biet Al-Magdis, Al-Quds Al-Sharif, and the old city of Jerusalem was known in the city of Zeitoun. Historically, their ancestral ancestors the Jebusites were the first to build and inhabited the city inthe fifth millennium BC. Now, the most important olive growing areas in Jerusalem: Beit Hanina, Beit Safafa, Kafr Aqaba, Shuafat, Jabel Mukaber, the French Hill, Jabal Al-Musharraf, Sheikh Jarrah, Wadi Al-Joz, Old City, Sur Bahir, Silwan, and Mount of olives (East Jerusalem) (Figure 11). Mount Olive or Tur is one of the oldest Palestinian villages in Jerusalem, located east of the Old City of Jerusalem, the Mount of Olives is the highest area in Jerusalem with a height of 826 meters above sea level and other areas in West Jerusalem have been under Israeli control since 1948, although they now control all areas of East and West Jerusalem. The area of olive trees (rainfed) Palestine was 893,721 dunums, and the productivity was 92kg/dunum, while the city of Jerusalem (East Jerusalem) was area of about 11,664 dunums, production (1400ton), and productivity was about 120kg/dunum during 2007/2008 [37], while the olive productivity in Jerusalem amounted to about 100.5kg / dunum during the year 1993-2012. The main olive varieties used in the Palestinian territories are: Shamali, Jabbah, Mantelino, Nabali Baladi, Nabali Mohsen, Shami, Suri, and Rumi [8,38].

Impact of temperature on olive

The temperature has a significant impact on the production [39], physiology [11-13], biology and sustainability of plants [40-42], where the olives growth need a cold period between 400-600 hours yearly, the starting temperature of olive growth is between 11-12°C, the optimal temperature for the growth of the olive tree ranging between 18 -20°C [4,5], the formation of clusters of fruitsat 15°C, flowers bloom starting at 19°C, the fruit contract occurs at 21°C, and the ripening temperature of fruits ranges from 35- 40°C, while the best storage temperature is between 10-15°C., as reported in the (Table 4).

Conclusion

Olive has a great economic importance that affects the Palestinian society as the largest and most important crop of fruit quarry in terms of area, number and production. The olives were found in Jerusalem and Palestine from more than 6,000 years ago, and it is believed that Palestine is the world’s olive origin and were mentioned in the Quran seven times. On the other hand, when we used a correspondence analysis (CA), observed that Jerusalem affected negatively by bioclimate factors as annual ombrothermicindex and simple continentality index during the years (1993-1997), whilst, the effect was positive during the years of study from (1997-2012), with a large proportion of the variance explained by axis F1 (96.81%), while axis F2 (2.88%) and axes F1and F2 (99.100%). However, in Jerusalem, the optimum of mean monthly temperature for increased olive production is between 15-200C, precipitation more than (600mm), with the result of annual ombrothermic index value more than 3.5, compensated thermicity index (210/210 to 350/350), simple continentality index between (15-20). Addition to olive production favor, where the environmental areas thermomediterranean to mesomediterranean of thermotype and dry to humid of ombrotype, and Jerusalem is belonging to mesomediterranean of thermotype and dry to sub humid of ombrotype.

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trdwriting · 7 years

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Moving Pains

Summary: After graduating college, Wilbur decides to move out of the Robinson household to live on his own. Carl doesn’t take the news very well.

Content Warnings: Two instances of foul language.

Portals: Fanfiction.net | AO3

A/N: Thanks to @that-guy-in-the-bowler-hat for requesting this. We were talking about headcannons after I wrote my 10th anniversary Meet The Robinsons fic and we got on the subject of Carl and Wilbur having an argument if Wilbur were to ever move out of the house. Thus, this fic was born!

This fic takes place in the same universe as my last one-shot “Revisited”, so George is making a return as Wilbur’s boyfriend. Hopefully, you all find him just as interesting as Wilbur, haha!

Wilbur uncapped the black Sharpie lying next to his leg and labelled the box he had recently taped shut “Winter Clothes”. He carried the box to the door and sat it alongside other cardboard boxes piled nearby. Packing up all his belongings was more of a transformative experience that he had originally anticipated. After many months of stomach aching, searching and discussion with his boyfriend, George, Wilbur had decided to move out of the Robinson household and live in a nearby apartment. Wilbur and his boyfriend both had secure jobs- him as a burgeoning video game developer and George as a staff writer for a local magazine. They had found an affordable apartment out closer to the city center. The floor space was rather small; there were two rooms and a bathroom. But it was cozy. George had moved the last of his belongings into their space yesterday. Wilbur was moving the bulk of his stuff today. His biggest worry with this transition had been his family. All his life, they had been more than supportive to pretty much anything he did (outside of abjectly stupid choices), but his entire extended family lived under one roof. For him to move out was against tradition. Because of this, Wilbur confessed his desire to live on his own as casually as possible over dinner, not knowing what to expect. The reactions were mixed. Uncle Joe sobbed, sucking his thumb vigorously (though he reacted that way if he didn’t eat on schedule, so that didn’t mean too much). His grandpa cheered for him, and his two uncles clapped him on the back. Aunt Petunia rolled her eyes and complained that his new “boy toy” was making him forget about the family. His mother and father wore indiscernible reactions on their faces. They looked at each other, exchanging information in just a glance. The rest of the family was swept up with mumbles.

His parents pulled him aside for a TalkTM, but ultimately, they encouraged him. His mother understood, as she had moved in with Cornelius after they had become a serious couple. “Should I be expecting an engagement announcement soon?” she asked him playfully, smirking at Wilbur. He just blushed and scrubbed at the back of his neck.

His father’s response was noncommittal. He said, “Well, as long as you are happy, I think you should do it. You are old enough to live on your own” and left the conversation at that. Wilbur could sense that his father was holding something else back, but he didn’t push the issue.

With his parent’s blessing, Wilbur was slowly gathering his belongings together. The rest of the family helped him out, making the actual move an easy affair. Uncle Art offered up his flying saucer as transportation, though Wilbur declined because he wasn’t sure if Uncle Art was allowed to use the company vehicle for personal purposes. Everyone else searched the house for anything that was Wilbur’s and brought it to him, so he could decide from there what to do with it.

Carl was the exception. Ever since he broke the news about his move, the robot hadn’t spoken much to Wilbur. Anytime they did talk, Carl fiddled with his hands and avoided eye contact at all costs. The conversations were brief and never about anything too serious. Whenever Wilbur asked him what was wrong, Carl would come up with some excuse and zip away before Wilbur had a chance to stop him.

Wilbur had started wrapping up his Captain Time Travel figures when he heard a knock on the door. Carl peered into Wilbur’s room, an unreadable expression on his face.

“Hey. Can I come in?” he asked, almost in a whisper. Wilbur beamed at his friend, standing up to meet him at the doorway.

“Yeah, of course, Carl.”

After Carl stepped inside, he narrowed his optics and looked away from Wilbur, tapping his metallic fingers against where his chin would be if his face weren’t a smooth cylinder shape. He turned back to Wilbur, ready to say something, but he paused, frowning. Wilbur arched an eyebrow, waiting for Carl to find his words.

“Look…Wilbur…uh…” Carl’s optics darted around the room, sticking onto places were his belongings were missing. “I…I’m really happy about you and George. You know, he’s…he’s a good kid. And I’m really proud of you! Super proud of all the things you’ve done and seriously…being your friend has been…such a gift.”

“…But?” Wilbur knew what was coming.

Carl gave him a deep sigh. “…but…I really don’t think you should move out.”

There it was. Although Carl hadn’t said as much, Wilbur had a feeling this is what Carl had been refusing to talk about. Wilbur swallowed back his frustration. “Why?” he asked, as he returned to packing.

“Well…you already have a home here! You don’t need to live away from us, you know?” Wilbur took another sheet of wrapping paper as he listened to Carl speak. “It’s a lot of work to live on your own, too. You have to pay your own bills and make your own food and- “

“Dude, I know. That’s why I want to move out. I’ve never done anything without the family before and…I want that to change. I think learning to be self-sufficient would be a good thing for me.” Wilbur loved his family-always had, always will. He was proud to be a Robinson, but sometimes he wished to exist as a more independent entity. People had associated his face with his father’s his whole life. He wanted an existence outside of that shadow, at least as much of one as he could.

Carl changed the subject. “Well…maybe George could move in here. We have plenty of room.”

“George wouldn’t want that. You know how he gets all shy when he is around too many people. The Robinsons would drive him nuts!”

When Carl didn’t say anything, Wilbur spoke up. “Look, Carl…George and I…We are in a serious relationship now. This isn’t one of my high school dates that I broke up with after three months. I’ve been with him for two years now.” Wilbur met Carl’s eyes. “I want to take the next step with him. Who knows, maybe some time down the line, we’ll get engaged.” His face blossomed red at the words. “But we won’t know if that’s the right move unless we spend time living with each other.”

Wilbur could hear the way Carl joints creaked as he moved and he imagined Carl was wildly gesticulating, the way he always did when he was passionately speaking. “So what? You don’t need to move away when you have a serious relationship. I haven’t moved out of this house once in all my years of dating!”

“All of your ‘dates’ lived here already, Carl! There was no need to move.”

“Well, if they didn’t live here, then I wouldn’t move anyway. A little biking never hurt anyone.”

Wilbur glared at Carl from the box he was packing. “Why are you bringing all of this up now? Right as I’m about to leave when you could’ve mentioned this to me at any point these last few weeks? I’m almost completely packed!”

Carl’s mouth flapped open and shut like a fish. He fidgeted. “I...I was having a hard time coming up with what to say. I mean, you seemed so dead-set on leaving, I…I only just now figured out all of my thoughts.”

“That’s such a lie.” Wilbur muttered.

“I’m telling you the truth!”

He felt all the seams of his composure break at the same time, his bile pouring out all at once. “You’ve never had a real relationship in your life! They were all with appliances!”

Wilbur regretted saying that as soon as it came out of his mouth. Carl stood there, shocked. If he had tear ducts, he would most certainly be crying. Before Wilbur could apologize, Carl left his room, sobbing.

The next day, Wilbur ate dinner with his family, his last dinner at home while living under the Robinson roof. The family found the occasion momentous enough for celebration, so they ate more dessert than usual- lava cakes, macaroons, apple pie and even ice cream sundaes. Wilbur could only stomach a bit of the lavish food. He probably would have had a larger apetite if a wad of guilt wasn’t weighing his stomach down. In between small bites of ice cream, he would glance at Carl, but Carl never met his eyes. He busied himself with serving the food and collecting any dirty dishes to be cleaned later. Wilbur glanced back down at his ice cream, suddenly feeling his stomach twist. He pushed the sundae away from him, the treat turning to ash on his tongue.

“Wilbur, why aren’t you eating your ice cream?” Cousin Lazlo asked, fiddling with his paint gun. “You love ice cream.”

At that comment, the whole table looked to Wilbur. He sat up a bit and cleared his throat. “Sorry, I’m just not that hungry right now. It was good, though.” He didn’t bother to look up. He knew his family were probably concerned, but Wilbur couldn’t be bothered to care.

Carl made no comment about Wilbur not finishing his favorite dessert. He merely stretched his arm over the length of the table and scooped the now- sundae soup without so much as a concern tilt of the head. As soon as his dishes were taken away, Wilbur got up from the table, heading back to his room.

“Wilbur?” He turned around when his dad called his name, hands in his pockets. Cornelius’s arms were folded over his chest. “Is everything okay?”

Wilbur drew a hand through his hair. “Yeah…yeah. I’m fine, Dad.” When Cornelius’s eyebrows didn’t lower, he realized that his father didn’t believe him at all. Not surprising. “Alright…no, I’m not fine.”

The creases in Cornelius’s face softened. He came closer to him and put a hand on his shoulder. “Do you want to talk about it?” he asked.

“No…I got it, Dad. I just got to talk to Carl and apologize for being a shithead,” he mumbled, ignoring the sour look Cornelius gave him at his curse. “Trust me. I know what to do.”

“Alright, son,” Cornelius pat Wilbur’s shoulder. “If you need anything, you know who to talk to.” Wilbur watched him walk away before heading to the kitchen, where Carl was planning out the next meal for the family. He had his head in the refrigerator when Wilbur cleared his throat. “Carl?”

He heard a metallic clank and a muffled ow. Carl emerged, rubbing the back of his head. “Jeez, Wilbur. You scared me!”

“Sorry.” Wilbur put his hands into his pockets again. “So, Carl. I just…came here to apologize for, y’know what I said to you and everything. It was pretty stupid of me and…and it wasn’t cool.” Wilbur studied Carl, waiting for the forgiveness that he was positive would be coming.

Instead, Carl just looked back at Wilbur blankly. “That’s it? That’s your apology.”

“What else am I supposed to say? I’m sorry for what I said about you.”

Carl huffed. “Do you think that’s going to make me feel better? Look, I’m sure you are sorry, bro, but I’m still hurt. What you said…….that was pretty painful. I’m not….I’m sorry, but I can’t forgive you just yet, okay?” Carl turned away from Wilbur, focusing on writing out a list of ingredients.

“Why not? I didn’t mean what I said!”

“You still said it.” Carl said bitterly. A rather awkward pause passed between them, heavy with Carl’s cold shoulder. Wilbur left without a word, getting Carl’s message.

Wilbur sat on the couch in his new apartment, playing a new video game he bought, hoping it would lift his spirits. Being stuck on the same jump wasn’t helping his mood. He groaned as the lost his last life to the same empty pit, tossing the controller to the side.

He cradled his face in his hands, so he only felt the couch dip when George took a seat next to him. “Couldn’t beat the level?” he asked. Wilbur felt a gentle hand rub between his shoulder blades.

He peered out from between his fingers at George. “No. I couldn’t.”

“Something up?” George had gotten good at reading his emotions over the years. Too good, sometimes.

Wilbur sat fully upright, the curve of his back pressed along the back of the couch. “It’s been a week and Carl still won’t talk to me. I’m starting to think that we might never be friends again.”

Wilbur heard George heave a sigh. “You’re exaggerating again, cariño.”

“Am I?” he huffed. “I apologized to him the day after we fought and yet he still won’t talk to me.” He stared up at the ceiling. “He told me he couldn’t forgive me yet, but I have a feeling maybe he never will forgive me! He’s never been this quiet before. Every time we’ve ever disagreed on something, we’ve always made up by the end of the day. But now…I don’t know if I can ever fix up what happened…What do I even do?”

A hand rested on Wilbur’s knee and he turned to look at George. To save what little resolve he had, he didn’t meet George’s hazel eyes. Wilbur couldn’t afford to get lost in them. He focused on his nose instead, narrowing on the minute shrinking and flaring of the nostrils.

It took him a moment to realize George was calling his name. “Sorry, what?” he asked, looking up, finally creating eye contact.

“I said, you need to give Carl some time. You obviously hurt him a lot. He is allowed not to forgive you, you know?”

Wilbur’s shoulder sunk. “Yeah, yeah…I know.” His head drooped with his sagging shoulders until George took his jaw in his hand and brought their eye contact together again.

“But…you and Carl have been friends since you were a baby. I have a feeling someone who put up with you as a bratty kid will be able to forgive you for being an idiot.”

Wilbur pouted. “I take offense with that.”

George’s mouth twitched to a smile, but his lips fell again. “Besides, I can’t really blame Carl for being upset about your moving out. He never liked me very much.”

What? He didn’t know what George was saying. Everyone loved him! “Dude, c’mon. Why would Carl not like y-“ The answer hit him in the face like a wet washcloth. “…You think he was jealous of us?”

George nodded. It was his turn to break eye contact. “He was okay with me when we were just friends, but…I think at some point he noticed that I had a crush on you and…well….”

Wilbur thought back to before they started dating, looking for evidence of George’s claims, but he came back empty-handed. “What do you mean? I don’t remember Carl acting weird around you. He might’ve while we were dating, but….”

“He was, cariño. Créeme.”

Wilbur wanted to believe his boyfriend was mistaken, but considering how wounded Carl had seemed after he had left the Robinson household didn’t give much hope to an alternative explanation. Wilbur took George’s hand. George turned to look at him, gently chewing the inside of his cheek like he always did when he was thinking.

Wilbur let go of his hand and wrapped it around his waist, resting his head against George’s wild chestnut hair. “You know, I’m really happy we moved in together.”

George tilted his head, but didn’t say anything. His wore a genuine smile on his lips, the kind that warmed his stomach the way a recent sip of hot chocolate did. “Me, too.”

Their lips met, gentle and loving. Wilbur cupped one of George’s cheeks and traced his cheekbone out with his thumb. After a few softer pecks, they separated. Wilbur smiled, moving his hand from his cheek to just along his jaw.

“Remember that one time when Carl caught us making out in my room?”

Wilbur snickered when George’s face immediately flushed, the red bleeding onto his neck. “Don’t remind me.” He grumbled in Spanish.

“I think he was more upset than you.” Wilbur said through a chuckle, replying in the same language.

George wrinkled his nose and squinted his eyes at Wilbur. He had shifted so their hips were touching. “Why are you bringing up the most embarrassing moment of my life?”

George’s expression was so cute that Wilbur needed a couple extra seconds to translate. “Well..” he put their foreheads together. “....because the way you kissed me was unforgettable.”

Wilbur’s heart kicked up a couple of notches when George smirked a bit and shook his head, chuckling. “You make it sound like that was the best kiss you ever had from me. We’ve shared an awful lot of them.”

Wilbur shrugged, trying to seem as unaffected as possible, even though George sometimes made it hard to breath. “Mind sharing some more with me?”

George replied by kissing Wilbur until his lips were red with them.

Dust floated in and out of the sliver of light spilling into Carl’s room as he shuffled through his belongings. As he quietly organized tools, he noticed a picture frame that had somehow wedged itself behind his desk. He retrieved it and almost immediately he wanted to return it to the dark corner of his room where it came from. A younger Wilbur, George and him smiled up from the frame, arms wrapped around each other. Carl was in the middle with Wilbur and George on either side. The two boys were no older than ten. Wilbur still had his rounded baby cheeks and George’s hair was long enough to almost cover his eyes.

Carl sighed (a purely vocal sigh, as he had no air to expel. It came out more like he had just discovered he could make sounds at all), cradling the frame in his palms. Wilbur, George and him had been in separable for Wilbur’s entire existence. The two boys had been in the same kindergarten class. From that point on, if he saw one of the boys, he guessed the other was not too far away. In the beginning, he had assumed their friendship would morph into a strong brotherly affection. That’s how Carl saw Wilbur anyway. (Technically, they shared a father.) When George was thirteen, their relationship shifted. They were still friends- always spending time together, but Carl could sense a tension between them- subtle enough to ignore, but strong enough to make their interactions minutely different. George would often cast glances at Wilbur when he wasn’t looking and Wilbur would do the same. He was surprised when Wilbur and George dated other people, expecting for a confession to come at any time. Finally, they started dating once Wilbur went off to college. And now…well…Wilbur was living with George.

Ah yes…He recalled the bitter argument he and Wilbur had before he left. Wilbur’s claim that he would never understand human romance still buzzed in his audio processors …Carl would admit that he was not the best with people, but Cornelius had programmed him well enough to see how much George meant to Wilbur. He understood the desire to stay around someone you cared about, especially someone you loved. He understood it, but it was still upsetting to see his best friend leave.

He recalled the apology Wilbur had given him the day after, while he was in the kitchen. Logically, Carl knew that Wilbur didn’t want to leave things on bad terms and probably felt horrible about what he said, but emotionally, Carl hadn’t been able to accept the apology. Wilbur’s word had felt shallow to him. Not because Wilbur didn’t honestly feel sorry for his actions, but more that Wilbur had expected Carl to forgive him. In some ways, that stung more than anything Wilbur had said. Couldn’t Wilbur see that he needed time to heal? That he couldn’t say that everything was okay when they clearly were not?

He was so lost in his melancholy that he almost didn’t hear the knock on his door. “Carl?”

Carl felt his gears shift, taken aback. He was not expecting to here Wilbur’s voice call to him. He set the photo down and opened the door. Wilbur cleared his throat. “Hey, Carl. Can we talk for a bit?”

Carl resisted the urge to leap at Wilbur and hug him. “Yeah.” He stepped out of his room completely. “Uh…so…Wilbur…”

Wilbur help up his hand. “Let me go first. Carl, I’m sorry about what I said to you before I moved out. It was…shitty of me and I feel really, really bad about it. I know that your relationships are important to you. I mean, you are one of the most understanding and loving guys I know. For me to insult you like that…well, I really messed up. And then when I expected you to be in a place to forgive me right after what happened… ” Wilbur was silent for a moment, letting what he said stew before continuing. “The reason I was so upset when we had that argument was because I was expecting you to support me, but I didn’t really think about your feelings and that a change like this is going to affect the people around me.”

Carl blinked, his lips twitching in a smile. He knew that Wilbur had a lot of pride, always thinking he knew what was best, that he had everything under control. For him to admit his weakness meant a lot. “I…I was really upset that you were moving out. Not because you were doing anything…you know, wrong…I just….” He sniffled. “…didn’t want you to grow up so fast. I mean, one minute you’re this…little buddy that wears space onesies…” He saw Wilbur roll his eyes at that comment. “…and the next, you’ve got a boyfriend! Next thing I know, you’re gonna be bringing home little buddies of your own and…. and…Oh, Wilbur! I’m gonna miss you so much!”

His resolve crumbled as he roped Wilbur into a hug. He didn’t notice he was squeezing too hard until Wilbur wheezed at him to let up, which he did. “Gosh, I’m so proud of you! I think you should name one of your kids after me.”

Wilbur’s cheeks were a deep shade of red and he coughed. Instead of responding, he clapped his hand on Carl’s spindly shoulder. “Thanks, pal. I’ll try to stop by more, okay?”

Carl nodded eagerly. “And I’ll stop by your new crib too. You have to show me around.”

“Maybe in a little while. There’s still some unpacking we have to do.”

The two of them chatted until Wilbur wrinkled his nose. “Something smells like it’s burning.”

Suddenly, Carl gasped and sped away to the nearest travel tube, yelling “NO! NOT THE APPLE PIE!” as he left. Wilbur chuckled and shook his head. He followed after him. Wilbur wasn’t going to miss out Carl’s apple pie for the world, even if it was a little burnt.

Translation Notes:

-cariño= (literally: affection) term of endearment; equivalent to honey or sweetie.

-Créeme.= Believe me.

I am not fluent in Spanish, so I appreciate any feedback. I want to be as accurate as possible.

Thank you for reading this work. I hope you all enjoyed it! Additional thank yous to @that-guy-in-the-bowler-hat for the request.

I have accounts on AO3 (TheRationalDove), Fanfiction.net (The Rational Dove) and Tumblr (tdrwritings). Want to request a fanfic? Send me an ask or PM!

-TheRationalDove

#Meet the Robinsons #fanfiction #that-guy-in-the-bowler-hat #wilbur robinson #oc #carl mtr #cornelius robinson #franny robinson #other misc robinsons #hurt/confort #family drama #more like a ittle slice of life scene #yeah #i can tag things

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diindjariin · 4 years

Text

Have You Ever Been in Love? (din djarin x reader)

Rating: t+

Word Count: 3.5k

Excerpt: “I like who you are now,” you say, hoping this will ease his pain, if only a little bit. The tilt of his head lets you know he’s smiling.

“And you?” he asks, “Have you ever been in love?”

“Oh yes,” you reply, breathless, and whisper for you in your head so many times you hope he can hear you.

Masterlist for this series

A/N: the screencap sucks again. roast me, please. if you’ve been following this series, this takes place right after soft hands, so it is not only the newest fic in the series, it is the newest chronologically. also, i am currently taking suggestions for the name of this series. feel free to help me out! as per usual, this fic had a quick once-over before being published so................expect errors. i admit wholeheartedly that i am the worst TM.

“We need money.”

“Okay. What’s the plan?”

“I’m going to get in contact with my old partner.” Your whole body stops and the only movement comes from your eyes, which slowly inch their way up his body and rest on the T of his visor.

“This isn’t up for negotiation,” he doesn’t sound angry, but he sounds sure. “I just want to discuss what to do with you and the kid.”

“You could drop us off on Sorgan,” you say casually, stroking the Child’s ears idly. “I’m sure Cara wouldn’t mind the company.”

“Are you mad at me?”

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