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jqhx40urw · 1 year

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erwinw · 2 months

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What are Colorimeters? Function, How It Works, and Maintenance

In a world where colors can speak louder than words, the unsung hero of precise hue measurement is the humble colorimeter, a device that unveils the silent language of colors with remarkable accuracy and simplicity. What are Colorimeters? A colorimeter is an instrument that plays a crucial role in the field of colorimetry, which is the science of measuring and analyzing the color of light that…

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#Colorimetric Analysis #Instrument Calibration #Spectral Measurement

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whats-in-a-sentence · 6 months

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Soil test kits (figure 11.10) use a 'universal indicator', which is a mixture of indicators that enable estimation of pH over a wide range.

"Chemistry" 2e - Blackman, A., Bottle, S., Schmid, S., Mocerino, M., Wille, U.

#book quotes #chemistry #nonfiction #textbook #colorimetric test #soil ph #ph test kit #universal indicator

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u7kkn4olxyb9 · 1 year

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f2xh2wd6cgi · 1 year

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taepomme · 6 months

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a little brush study with macca!!! and other colorimetric test

#the beatles #beatles #paul mccartney #linda mccartney #beatles art #Ram #john lennon #george harrison #ringo starr

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kevink101 · 2 years

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RS1 Colorimetric Analyzer

#RS1 Colorimetric Analyzer

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peoneys · 1 year

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“She freezes, but Solas keeps moving, and she has to move with him so she doesn’t stumble. Her feet are bare, the only familiar thing other than Solas’ presence besides her. Except he is not familiar at all, no hint of the humble apostate. She is out of place, plain and small. [...]

Here is another person to talk to, another introduction made. They look at her briefly, but with no challenge. They are there to talk to Fen’Harel.

That is what they call him, again and again. Her mind screams. Unreal. Unreal. Unreal. [...]”

That drawing was largely inspired by “Temporal Arrangements”’ first chapter by thevikingwoman ahaha

Extremely proud of this one,even tho I battled to death with the iPad’s colorimetric setting..I just hope the colors will look okay on your screens 🌟

#fanart #my art #art #dragon age #dragon age inquisition #solas #lavellan #solavellan #seth lavellan #dai #lune #oc

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canmom · 5 months

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Have you seen some of the new HDR stuff on KDE plasma 6? Still early days, but very exciting!

https://zamundaaa.github.io/wayland/2023/12/18/update-on-hdr-and-colormanagement-in-plasma.html

oh that is interesting! sounds very promising.

“SDR Color Intensity” is inspired by the color slider on the Steam Deck. For sRGB applications it scales the color gamut up to (at 100%) rec.2020, or more simply put, it makes the colors of non-HDR apps more intense, to counteract the bad gamut mapping many HDR displays do and make colors of SDR apps look more like when HDR is disabled

I guess this goes some way to answering why colour accuracy for SDR content was so dodge when I turned on HDR on windows (which lacks such a slider, just a brightness slider). "the bad gamut mapping many HDR displays do" is big oof but also explains a lot. the mathematics for colour space transformation is pretty well-defined, so if hardware is implementing them incorrectly, that's really frustrating. older screens are working with standards that predate the idea of colour management, but now we're bringing in all these new display signalling standards (PQ and all that), it seems like a golden opportunity to do things properly this time... so of course we don't lmao.

the colorimeter I got has an option for high brightness/HDR screens, but I haven't really tested it yet. 'measure your screen colorimetrically and compensate in software' works decently well in SDR land, but I'm not sure what happens in HDR. I haven't played any HDR-enabled games since I got this colour accuracy bug, so I haven't had cause to try and figure out the ins and outs of it yet.

it seems like KDE intend to be a lot less black-box about it than Microsoft, which is very welcome.

#canmom vs colour #linux #computer science #all this is sutff artists tend to be a lot more concerned about than our audiences in general...

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rohanisblog · 2 months

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Residual Chlorine Meters Market Analysis by Geographical Regions, Type and Application and Forecast to 2031

Global Residual Chlorine Meters Market is estimated to witness a rise in revenue from US$ 330.2 Mn in 2021 to US$ 527.3 Mn by 2030. The market is registering a CAGR of 5.3% during the forecast period 2022-2030. Moreover, in terms of volume, the global residual chlorine meters market is expected to project a CAGR of 4.9% during the forecast period 2022-2030.

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The report identifies and analyzes the key players in the Residual Chlorine Meters Market. By understanding the competitive landscape, businesses can benchmark their performance and develop effective strategies to stay ahead.

The key players in the Global Residual Chlorine Meters Market are Horiba, DKK-Toa Corporation, Hanna Instruments, Hach Company, Tanita, Automated Water & Effluent Ltd., Yokogawa Electric Corporation, Analyticon Biotechnologies GmbH, Rakiro Biotech Systems Private Limited, BOQU Instruments, ABB Ltd., Lohand Biological, Christian Bürkert GmbH & Co. KG and Extech Instruments among others.

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By Solution segment of the Global Residual Chlorine Meters Market is sub-segmented into:

Equipment Consumables Reagent Kits Reagent Tablets/Powders Color Wheel Comparator Kits Test Tube Comparator Kits Pool Test Kits Test Strips Electrodes Accessories

By Measurement segment of the Global Residual Chlorine Meters Market is sub-segmented into:

Offline (DPD Colorimetric Detection) Online/Continuous (Amperometric Sensors)

By Form segment of the Global Residual Chlorine Meters Market is sub-segmented into:

Wall-mount Handheld/ Portable Pen Style

By Display segment of the Global Residual Chlorine Meters Market is sub-segmented into:

LED Display LCD Display

By Application segment of the Global Residual Chlorine Meters Market is sub-segmented into:

Water Purification Plant Industrial Municipal Food and Beverages Desalination Laboratory Use Others

By Region segment of the Global Residual Chlorine Meters Market is sub-segmented into:

North America The U.S. Canada Mexico Europe The UK Germany France Italy Spain Poland Russia Rest of Europe Asia Pacific China India South Korea Japan Australia & New Zealand ASEAN Rest of Asia Pacific Middle East & Africa (MEA) UAE Saudi Arabia South Africa Rest of MEA South America Argentina Brazil Rest of South America

The company has partnered with industry-leading experts, utilizing cutting-edge research methodologies and data analysis techniques to provide a reliable and accurate market report. With a focus on delivering actionable insights, this report is an indispensable tool for businesses looking to navigate the complexities of the Residual Chlorine Meters Market and unlock new growth opportunities.

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#Residual Chlorine Meters Market #Size #Share #Trends

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sybaritick · 3 months

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ive had "guy who has had an unrealistic number of different jobs" in my bio for ages but just now i was reminded about when i interned in an immunology lab and was joking about how, look yes im primarily a programmer but things like that (the bio lab background) and that i've worked on Arduino stuff/actual electronics at least a little bit, even though i was a software guy on that project and not a hardware guy (this was in the shark tagging lab), this is all because being solely a programmer is not enough to actually satisfy my obsession w cybernetic brain implants (for straightforwardly villainous purposes).

you also have to be a neuroscientist and an biomedical engineer. ye i am a programmer because it's the easiest to do without additional formal education but the other stuff i just have to run around collecting relevant experience from the various gigs i've had. so it's cool i've spent time in a biology lab and done PCR and colorimetric assays and gel electrophoresis and that gives me a BIT of useful knowledge for certain stuff, but i really do need to get more neuroscience background. or need to find someone who does and doesn't ask a lot of questions.

#cal txt #crypto-gortashposting if im being real here #lmao

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weeping-laurels · 5 months

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I’ve struggled with iron deficiency anemia for years and tend to bruise very easily. I always find random bruises on my skin and since I’m very strange and love documenting everything, have kept a journal for years estimating the ages and healing progression of all my bruises, cuts, and burns. My favorite pastime is lining up my bruises with tables or couch edges to see where and how I managed to get them…

Our town coroner even gave me this colorimetric scale for assessing bruising (it’s like an L shaped plastic ruler; small towns amirite?) and I use it as a bookmark!

Point of the story, is I can only imagine what fun I’d have finding bruises and love bites all over my body the day after a rather rough sex session. Ideally I’d need help counting them all. We’d celebrate them healing by making more in new, creative places and make a game out of trying to control their shapes and sizes before they develop. I want a heart bruise on my thigh!

Too niche?

#weeping laurels #bruises #rough kink #nsft #bi nsft #I need to go tag shopping

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whats-in-a-sentence · 7 months

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Less accurate values can be obtained using pH paper (figure 11.6), while blue and red litmus paper is used only in a qualitative manner to determine whether a solution is acidic or basic.

"Chemistry" 2e - Blackman, A., Bottle, S., Schmid, S., Mocerino, M., Wille, U.

#book quotes #chemistry #nonfiction #textbook #ph test #ph paper #colorimetric test #litmus paper #litmus test #acidic #basic

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thebotanicalarcade · 7 months

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Fluorescence reveals hidden details in flowers that attract pollinators.

Certain areas of the flower including some pollen and nectar are fluorescent as well, which could potentially aid in the pollination of flowers.

A yellow wood anemone (Anemone ranunculoides) was imaged with ImageQuant™ 800 using (A) colorimetric and (B) fluorescent imaging modes at UV (red), Cy™3 (green), Cy™5 (yellow), and IR long (blue).

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primamedikatama · 8 months

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kendricklabsinc · 6 days

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A Step-by-Step Guide to Performing Western Blotting Protein

Western blotting is a powerful and widely used technique in molecular biology and protein analysis. In this comprehensive guide provided by Kendrick Labs Inc., we will walk you through the step-by-step process of performing Western blotting with precision and accuracy. From sample preparation and protein extraction to antibody incubation and data interpretation, each key stage of the Western blotting workflow will be detailed to help researchers and scientists achieve reliable results in protein detection and analysis. Follow along to master the fundamentals of Western blotting and enhance your experimental skills in the laboratory.

What is Western Blotting?

If science had a dating app, Western blotting would be the swiping right participant. It's a technique used to detect specific proteins in a sample, kind of like finding Waldo in a sea of stripes.

Applications of Western Blotting

Western blotting is the undercover agent of the lab world, utilized in various fields like molecular biology, immunology, and biochemistry. It helps researchers identify proteins, study protein expression levels, and even solve mysteries like a true detective.

Overview of Western Blotting Process

Key Steps in Western Blotting

Think of Western blotting as a protein talent show, where the key steps include protein separation, transfer to a membrane, blocking, primary and secondary antibody incubation, and finally, detection.

Importance of Protein Detection

Proteins are the Beyoncé of cells - they run the show. Detecting and quantifying proteins through Western blotting helps scientists understand cellular processes, biomarker discovery, and even evaluate treatment responses.

Sample Preparation and Protein Extraction

Tissue or Cell Lysis

It's like breaking into a safe, but a scientific one. Tissue or cell lysis is the process of breaking open cells to release proteins, akin to cracking open a delicious protein-filled piñata.

Protein Quantification Methods

Quantifying proteins is like counting the number of cookies in a jar – it's crucial for accurate results. Various methods like Bradford assay or BCA protein assay help determine protein concentrations for the Western blotting magic show.

Gel Electrophoresis and Protein Separation

Preparation of SDS-PAGE Gel

Creating an SDS-PAGE gel is like laying down the red carpet for proteins. It provides a platform for protein separation based on size, ensuring each protein gets its moment in the spotlight.

Running the Gel and Protein Separation

Running the gel is the protein marathon – proteins move through the gel at different speeds based on size, creating distinct bands like a molecular fingerprint. This step sets the stage for the Western blotting revelation to come.

Protein Transfer Techniques

Overview of Protein Transfer Methods

When it comes to transferring proteins from a gel to a membrane, Western blotting offers a variety of methods to suit your needs. From traditional wet transfers to speedy semi-dry transfers, there's a method for every protein aficionado.

Optimizing Transfer Efficiency

For a successful protein transfer, ensure your transfer buffer is fresh, your voltage is just right (not too high, not too low), and your proteins are evenly transferred. Remember, even proteins deserve a smooth journey from gel to membrane!

Antibody Incubation and Detection

Primary and Secondary Antibody Incubation

Like a perfectly crafted sandwich, the primary antibody is the flavorful filling that binds specifically to your protein, while the secondary antibody adds the visual flair for detection. Don't forget to incubate with care and wash away any unbound antibodies.

Visualization of Protein Bands

Once your antibodies have worked their magic, it's time to uncover those elusive protein bands. Whether you opt for chemiluminescence, fluorescence, or colorimetric detection, make sure your proteins are ready for their close-up moment.

Data Analysis and Interpretation

Image Acquisition and Analysis Software

Now that your protein bands have posed for the camera, it's time for some data analysis fun! Choose your favorite image analysis software and let it work its magic to quantify those bands and normalize your data. Numbers never looked so good!

Quantification and Normalization of Protein Bands

When it comes to interpreting Western blot data, remember the golden rule: quantify, quantify, quantify! Normalize your protein bands to housekeeping genes or total protein levels for an accurate representation of your results. It's all about that protein balance!

Troubleshooting and Common Pitfalls

Addressing High Background Noise

Ah, the pesky background noise that loves to photobomb your protein bands. Combat this common culprit by tweaking your blocking buffer, optimizing antibody concentrations, or just giving that membrane a good wash. Say goodbye to noisy neighbors!

Strategies for Weak Signal Detection

When your protein bands are feeling a bit camera-shy, fear not! Boost your signal with a longer exposure time, optimize your antibody dilutions, or try out signal amplification techniques. A little patience and creativity can go a long way in revealing those shy proteins.

In conclusion, mastering the art of Western blotting is essential for researchers and scientists looking to delve deeper into the realm of protein analysis. By following the detailed steps and guidelines outlined in this guide provided by Kendrick Labs Inc., you can enhance your understanding and proficiency in Western blotting techniques. With practice and attention to detail, you can harness the power of Western blotting to unlock valuable insights into protein expression and function, furthering your research endeavors and contributing to advancements in the field of molecular biology.

Original Sources: https://kendricklabs.blogspot.com/2024/05/a-step-by-step-guide-to-performing.html

#Western Blotting Protein #Immunoblot Western Blot

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