YHS as things I have said #1

Sam: bad thoughts lead to poking your enemies with the stabby stabby ~~~~~~~~~~~~~~~~~ Taurtis: No compass, we wander around aimlessly like men! or Taurtis: I love eating raw cookie dough, Salmonella adds extra flavor ~~~~~~~~~~~~~~~~~~~~~~~ Grian: Lifehack! You can’t be a self simp if you despise yourself!  Or Grian: ha imagine having low self esteem! I can relate and it sucks :,) ~~~~~~~~~~~~~~~~~~~~~~~ Dom: I will T-Pose on your grave, and this time It will NOT be in respect! or Dom: You can’t hide from the gay! ~~~~~~~~~~~~~~~~~~~~~~ J: I control when I want to be smart or not ~~~~~~~~~~~~~~~~~~~~~~ Pepe: You will die from your stupidity and somehow I will die before you or Pepe: Listen man I only have one braincell, and it is dried up from me sobbing ~~~~~~~~~~~~~~~~~~~~~~ Pie: That fire is colored like the bi flag, that speaks to me on an emotional level

SHANK3 Regulates Intestinal Barrier Function Through Modulating ZO-1 Expression Through the PKCε-dependent Pathway.

SHANK3 Regulates Intestinal Barrier Function Through Modulating ZO-1 Expression Through the PKCε-dependent Pathway.

Inflamm Bowel Dis. 2017 Oct;23(10):1730-1740

Authors: Wei SC, Yang-Yen HF, Tsao PN, Weng MT, Tung CC, Yu LCH, Lai LC, Hsiao JH, Chuang EY, Shun CT, Ni YH, Xavier RJ, Podolsky DK, Yen JJY, Wong JM

Abstract BACKGROUND: The integrity of the gut barrier in patients with inflammatory bowel disease is known to be impaired but the exact mechanisms remain mostly unknown. SHANK3 mutations are associated with autism, and patients with autism are known to have higher proportions of inflammatory bowel disease. Here, we explore the role of SHANK3 in inflammatory bowel disease, both in vivo and in vitro. METHODS: Dextran sulfate sodium colitis was induced in SHANK3 knockout mice. Transepithelial electrical resistance, paracellular permeability, and Salmonella invasion assays were used to evaluate epithelial barrier function, in vitro and in vivo. Expression of tight junction proteins, protein kinases, and MAP kinase phosphorylation changes were analyzed by immunoblotting after overexpression or knockdown of SHANK3 expression. SHANK3 expression in intestinal tissue from patients with Crohn's disease was analyzed by quantitative polymerase chain reaction and immunohistochemistry. RESULTS: SHANK3 knockout mice were more susceptible to dextran sulfate sodium. SHANK3 knockout resulted in a leaky epithelial barrier phenotype, as demonstrated by decreased transepithelial electrical resistance, increased paracellular permeability, and increased Salmonella invasion. Overexpression of SHANK3 enhanced ZO-1 expression, and knockdown of SHANK3 resulted in decreased expression of ZO-1. Regulation of ZO-1 expression by SHANK3 seems to be mediated through a PKCε-dependent pathway. SHANK3 expression correlated with ZO-1 and PKCε in colonic tissue of patients with Crohn's disease. CONCLUSIONS: The expression level of SHANK3 affects ZO-1 expression and the barrier function in intestinal epithelial cells. This may provide novel insights in Crohn's disease pathogenesis and treatment.

PMID: 28906292 [PubMed - in process]

via pubmed: autism http://ift.tt/2xpBlNC

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