Daughters of Darkness (Harry Kümel, 1971)

Cast: Delphine Seyrig, John Karlen, Danielle Ouimet, Andrea Rau, Paul Esser, Georges Jamin, Joris Collet, Fons Rademakers. Screenplay: Pierre Drouot, Harry Kümel. Cinematography: Eduard van der Enden. Art direction: Françoise Hardy. Film editing: Denis Bonan, August Verschueren. Music: François de Roubaix. 

Daughters of Darkness is a horror movie loaded with style, featuring Delphine Seyrig in what feels a bit like a camp sendup of her mysterious woman in Last Year at Marienbad (Alain Resnais, 1961). It's about a couple of newlyweds who come under the spell of a woman who claims to be the Countess Elizabeth Báthory, the 17th-century Hungarian serial killer whose story became a staple of vampire lore. Seyrig is a treat as the undead countess, slinking about in a variety of outfits that include a silver-lamé dress which cinematographer Eduard van der Enden films with a star filter to jazz up its dazzle. It's all very creepy and over-the-top, but unfortunately, it also leans heavily on the old trope of queerness as decadence, a too-common feature of films from its era. The husband, Stefan (John Karlen), seems to be the boy toy of a man he calls “Mother,” which causes some tension with Valerie, his new wife (Danielle Ouimet), who keeps insisting that he call his mother to tell her that they're coming for a visit. Stefan turns out to be a bit of a sadist, driving Valerie into the arms of the countess. Karlen seems to have landed the role of Stefan in part because he was already known from another vampire story, the TV series Dark Shadows. Today, he's probably mostly remembered as Harvey, the husband of Tyne Daly's Lacey, on the series Cagney & Lacey. 

- Where do you think you’re going Valerie? - Let me go. You’re hurting me. Will you let me go? - No. Not till you’ve answered me. - I’m going back to Stefan. That’s what you wanted, isn’t it? I’ll tell him. - You’ll tell him what? That you understand him now? That you understand his kind of love? - Stefan loves me, whatever you may think. - "Stefan loves me, whatever you may think.” Of course he does. That’s why he dreams of making out of you what every man dreams of making out of every woman. A slave, a thing, an object for pleasure. So you despise me. So I disgust you. Come. I’ll show you what men are really made of. Every man. Yours.

Daughters of Darkness (Les lèvres rouges), Harry Kümel (1971)

Les lèvres rouges (Harry Kümel, 1971).

@ Patje0001: @Caroline_syntje Denktank?? M.a.w. we hebben weer iets ineen gestoken zodat bepaalde personen er een mandaatje of een postje bij kunnen nemen.

Denktank?? M.a.w. we hebben weer iets ineen gestoken zodat bepaalde personen er een mandaatje of een postje bij kunnen nemen.

— Patje Verschueren (@Patje0001) August 15, 2018

Wʜᴀᴛ ᴍᴀᴋᴇs you sᴍɪʟᴇ﹖ "The picture has been taken during a sunny day in August 2015. That day we had a nice party for the baptism of Oliver in Bornem, Belgium. The big girl on the picture is Annick Verschueren, one of my best friends. In 2012 she asked me to be the godmother of her daughter Eloise the girl I'm holding in my arms. Nothing but smiles on the picture because it's all about family." 🎉🙏😃❤ @virginiedeklippel from #Belgium 🇧🇪 (at Belgium)

Swarm Intelligence-Enhanced Detection of Non-Small-Cell Lung Cancer Using Tumor-Educated Platelets

Publication date: 14 August 2017 Source:Cancer Cell, Volume 32, Issue 2 Author(s): Myron G. Best, Nik Sol, Sjors G.J.G. In ‘t Veld, Adrienne Vancura, Mirte Muller, Anna-Larissa N. Niemeijer, Aniko V. Fejes, Lee-Ann Tjon Kon Fat, Anna E. Huis In ‘t Veld, Cyra Leurs, Tessa Y. Le Large, Laura L. Meijer, Irsan E. Kooi, François Rustenburg, Pepijn Schellen, Heleen Verschueren, Edward Post, Laurine E. Wedekind, Jillian Bracht, Michelle Esenkbrink, Leon Wils, Francesca Favaro, Jilian D. Schoonhoven, Jihane Tannous, Hanne Meijers-Heijboer, Geert Kazemier, Elisa Giovannetti, Jaap C. Reijneveld, Sander Idema, Joep Killestein, Michal Heger, Saskia C. de Jager, Rolf T. Urbanus, Imo E. Hoefer, Gerard Pasterkamp, Christine Mannhalter, Jose Gomez-Arroyo, Harm-Jan Bogaard, David P. Noske, W. Peter Vandertop, Daan van den Broek, Bauke Ylstra, R. Jonas A. Nilsson, Pieter Wesseling, Niki Karachaliou, Rafael Rosell, Elizabeth Lee-Lewandrowski, Kent B. Lewandrowski, Bakhos A. Tannous, Adrianus J. de Langen, Egbert F. Smit, Michel M. van den Heuvel, Thomas Wurdinger Blood-based liquid biopsies, including tumor-educated blood platelets (TEPs), have emerged as promising biomarker sources for non-invasive detection of cancer. Here we demonstrate that particle-swarm optimization (PSO)-enhanced algorithms enable efficient selection of RNA biomarker panels from platelet RNA-sequencing libraries (n = 779). This resulted in accurate TEP-based detection of early- and late-stage non-small-cell lung cancer (n = 518 late-stage validation cohort, accuracy, 88%; AUC, 0.94; 95% CI, 0.92–0.96; p < 0.001; n = 106 early-stage validation cohort, accuracy, 81%; AUC, 0.89; 95% CI, 0.83–0.95; p < 0.001), independent of age of the individuals, smoking habits, whole-blood storage time, and various inflammatory conditions. PSO enabled selection of gene panels to diagnose cancer from TEPs, suggesting that swarm intelligence may also benefit the optimization of diagnostics readout of other liquid biopsy biosources.

Graphical abstract

Teaser

Best et al. use particle-swarm optimization algorithms and RNA-seq of tumor-educated platelets from patients to generate RNA sets capable of identifying patients with non-small-cell lung cancer, including those having early stage, from individuals without cancer, including those having inflammatory conditions. http://ift.tt/2uU2Evs