Lmao what happened to ur HPLC

I wish I fuckin knew what was wrong with it. Right now it keeps blowing past the safe pressure limit, and we first though it was maybe the tubing, because it was happening when it would pull from those specific slots, but we changed out all the tubing and no dice. New running theory is that something's up with the injector pump, because the other HPLC's injector pump is flat out refusing to do it's job. We don't have any spares though, and the repair guys can't come in until mid April. My research partner and I are clawing our fucking eyes out over this, because we use the HPLC for everything, so we're at a standstill for now. The only other thing we can work on atm is microtox, which is tedious as hell and I hate it.

The HPLCs have been kindof on the fritz since last spring. There was this crazy power outage on our campus and the surrounding town because of some fuck up at the power plant (I was in our science building at the time and there were people trapped in the elevator). Apparently our HPLCs were the only things in the instrument lab that weren't connected to surge protectors, idk why that is, but that's about when they started having trouble so I think they got screwed up by the surge and that it's a bigger problem than just the injector and tubing.

We thought we cleared up the original original problems from last spring, but apparently our professor never actually had anyone come in to professionally check on the HPLCs after the power surge (Stingy bastard, I know we have the grant money to do it). So yeah, they've been on and off for the better part of a year now. The past three month though have been the worst for it though. Before, we could get decent readings, we just had to be delicate. Now we cant even use them reliably.