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ao3feed-stucky · 4 years

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by CrushedRose, kocuria

Time is a wonderful teacher, but in the end, kills all his students.

Several months after his dramatic breakdown in "The Power of Five Seconds" Bucky is finally on a road to recovery. He becomes part of a community in Wakanda, and starts to build a life with his new-found family around him. He and Steve are slowly and painfully rebuilding the trust lost after the events of Endgame. So of course, there must be another storm gathering just beyond the horizon. Disclaimer: We don't own Marvel or have any association with it. This is just our version of the story.

Words: 4960, Chapters: 1/44, Language: English

Series: Part 3 of The Value of Time

Fandoms: Captain America (Movies), The Avengers (Marvel Movies)

Rating: Teen And Up Audiences

Warnings: No Archive Warnings Apply

Categories: Multi

Characters: James "Bucky" Barnes, Steve Rogers, Sam Wilson (Marvel), Shuri (Marvel), Stephen Strange, Original Characters, Original Female Character(s), Original Male Character(s), Original Female Character(s) of Color, Original Male Character(s) of Color, Rebecca Barnes Proctor, Winifred Barnes, Sarah Rogers

Relationships: James "Bucky" Barnes/Steve Rogers, James "Bucky" Barnes & Steve Rogers, James "Bucky" Barnes & Sam Wilson, James "Bucky" Barnes/Original Character(s), James "Bucky" Barnes & Wanda Maximoff, James "Bucky" Barnes & Shuri

Additional Tags: Not A Fix-It, Post-Canon Fix-It, Hurt/Comfort, Emotional Hurt/Comfort, Avengers: Endgame (Movie) Spoilers, Post-Avengers: Endgame (Movie), Avengers: Endgame (Movie) Compliant, Sort of a fix it, bucky moving on, Steve Rogers Feels, Steve Rogers Needs a Hug, Bucky Barnes Feels, Bucky Barnes Needs a Hug, Bucky is happy, Sam Wilson Is a Good Bro, Hurt Bucky Barnes, bucky and sam are good friends, bucky has an amazing support system, steve learns his lesson, steve takes the stupid, Protective Bucky Barnes, bucky has social media, Not Steve Friendly, although stucky is endgame, it takes a while, Don't copy to another site, Betaed, Awesome Shuri (Marvel), Awesome Wanda Maximoff

#Bucky #AO3 works tagged 'James Barnes/Steve Rogers'#ao3feed #stucky #steve rogers #bucky barnes #c

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juniperpublishersmicrobiology · 5 years

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Extreme Cold Environments: A Suitable Niche for Selection of Novel Psychrotrophic Microbes for Biotechnological Applications

Authored by Ajar Nath Yadav

Editorial

The microbiomes of cold environments are of particular importance in global ecology since the majority of terrestrial and aquatic ecosystems of our planet are permanently or seasonally submitted to cold temperatures. Earth is primarily a cold, marine planet with 90% of the ocean's waters being at 5°C or lower. Permafrost soils, glaciers, polar sea ice, and snow cover make up 20% of the Earth's surface environments. Microbial communities under cold habitats have been undergone the physiological adaptations to low temperature and chemical stress. Recently, these communities have attained the focus of applied research not only in terms of biotechnological prospects but also to understand the use of primitive analogues of biomolecules existed during early Earth environments [1,2]. The microbiomes of cold environments have been extensively investigated in the past few years with a focus on culture dependent and culture-independent techniques. Cold-adapted microorganisms have been reported from Antarctic sub-glacial, permanently ice-covered lakes, cloud droplets, ice cap cores from considerable depth, snow and ice glaciers [3-6]. Many novel microbes have been sort out from cold environments including Halobacterium lacusprofundi [7], Sphingobacterium antarcticus [8], Octadecabacter arcticus [9], Hymenobacter roseosalivarius [10], Cellulophaga algicola [11], Flavobacterium frigidarium [12], Oleispira antarctica [13], Flavobacterium psychrolimnae [14], Psychromonas ingrahamii [15], Exiguobacterium soli [16], Pseudomonas extremaustralis [17], Cryobacterium roopkundense [18], Sphingomonas glacialis [19], Pedobacter arcticus [20], Sphingobacterium psychroaquaticum [21], Lacinutrix jangbogonensis [22], Massilia eurypsychrophila [23], Glaciimonas frigoris [24] and Psychrobacter pocilloporae [25]. There are several reports on whole genome sequences of novel and potential psychrotrophic microbes [26,27].

The novel species of psychrotrophic microbes have been isolated worldwide and reported from different domain archaea, bacteria and fungi which included members of phylum Actinobacteria, Proteobacteria, Bacteroidetes, Basidiomycota, Firmicutes and Euryarchaeota [7-25]. Along with novel species of psychrotrophic microbes, some microbial species including Arthrobacter nicotianae, Brevundimonas terrae, Paenibacillus tylopili and Pseudomonas cedrina have been reported first time from cold deserts of NW Himalayas and exhibited multifunctional plant growth promoting (PGP) attributes at low temperatures [5]. In a study by Yadav et al. [6], the microbial species Alishewanella sp., Aurantimonas altamirensis, Bacillus baekryungensis, B. marisflavi, Desemzia incerta, Paenibacillus xylanexedens, Pontibacillus sp., Providencia sp., P. frederiksbergensis, Sinobaca beijingensis and Vibrio metschnikovii have been reported first time from high altitude and low temperature environments of Indian Himalayas. Wheat associated psychrotrophic bacteria Arthrobacter methylotrophus and Pseudomonas rhodesiae have been reported first time from wheat growing in North hills zone of India [28]. In a specific search of economically important Bacillus and Bacillus derived genera (BBDG) at low temperature, Various BBDG such as Bacillus psychrosaccharolyticus, B. amyloliquefaciens, B. altitudinis, B. Muralis, Paenibacillus tylopili, P. pabuli, P. terrae and P lautus with efficient PGP attributes have been reported first time by Yadav et al. [29].

Prospecting the cold habitats has led to the isolation of a great diversity of psychrotrophic microbiomes. The bacterialAdv Biotech & Micro 2(2): AIBM.MS.ID.555584 (2017)diversity from the cold environment could serve as a database for selection of bio-inoculants with PGP ability and could be used for improving the growth and yield of crops grown at high altitudes with prevailing low temperatures [30-33]. Psychrotrophic PGP microbes have been shown to promote plant growth either directly by biological N2-fixation; solubilization of minerals such as phosphorus, potassium and zinc; production of siderophores and plant growth hormones (Indole acetic acid and gibberellic acid) or indirectly, via production of antagonistic substances by inducing resistance against plant pathogens [29,34,35]. The psychrotrophic PGP microbes can have an impact on plant growth providing the plant with compound(s) of microbial origin for facilitating the uptake of nutrients from the environment. Psychrotrophic PGP microbes were found in several genera, including Arthrobacter, Bacillus, Brevundimonas Burkholderia, Pseudomonas, Citricoccus, Exiguobacterium, Flavobacterium, Janthinobacterium, Kocuria, Lysinibacillus, Methylobacterium, Microbacterium, Paenibacillus, Providencia and Serratia [3538]. Among these taxa, Pseudomonas and Exiguobacterium has been the best characterized for PGP at low temperatures [38,39]. There are several studies have demonstrated the benefits of PGP microbes on the growth and yield of different crops at different climates, soils, and temperatures. The use of PGP microbes improves plant growth by supplying plant nutrients, which can help sustain environmental health and soil productivity.

Psychrophilic/psychrotolerant microbes are important for many reasons, particularly because they produce cold- active enzymes. The enzymes from psychrophiles have become interesting for industrial applications, partly because of ongoing efforts to decrease energy consumption. These cold-active enzymes provide opportunities to study the adaptation of life to low temperature and the potential for biotechnological exploitation [2,40]. Most of the work that has been conducted on psychrophilic bacteria focused on cold-active enzymes such as amylase, protease, lipase, pectinase, xylanase, cellulase, P-glucosidase, p-galactosidase and chitinase [40]. Cold- active enzymes are produced by psychrophilic microbes namely, Acinetobacter, Aquaspirillium, Arthrobacter, Bacillus, Carnobacterium, Clostridium, Cytophaga, Flavobacterium, Marinomonas, Moraxella, Moritella, Paenibacillus, Planococcus, Pseudoalteromonas, Pseudomonas, Psychrobacter, Shewanella, Vibrio and Xanthomonas [27,41,42]. Psychrophilic microbes can be applied for biodegradation of agro wastes at low temperatures. Shukla et al. [43], have developed psychrotrophic microbial consortium of Eupenicillium crustaceum, Paceliomyces sp., Bacillus atropheus and Bacillus sp., for its potential applications towards degradation of agri-residues and conversion to a value added product like compost for enhancing soil fertility and decreasing environmental pollution caused by burning of agro-wastes. Psychrotrophic microbes produced anti-freezing compounds (AFCs) at low temperatures [1,27]. The AFCs are useful in cryosurgery and also in the cryopreservation of isolated organs, cell lines, tissues and whole organisms. In food industry, anti-freezing proteins (AFPs) can be used to improve the quality of frozen food. Improved cold tolerance in fishes has been achieved in some cases by direct injection of AFPs and in another case by transgenic expression of an AFPs.

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Conclusion

Cold-adapted microbes could be utilized for understanding adaptation at low temperatures, as they produce cold-active enzymes, fatty acids, carotenoids, cold acclimation proteins, cryoprotectants and anti-freezing proteins under extreme conditions of low temperature. Cold-active enzymes have applications in industry like those manufacturing cleaning agents or in leather processing. The other applications could be for bio-degradation of xenobiotic compounds in cold climes, food processing (bakery, cheese manufacture and fermentation) and molecular biology (heterologous gene expression). Cold- adapted microbes have attracted the attention of the scientific community due to their ability to promote plant growth and produce cold-active enzymes, with potential biotechnological applications in a broad range of industrial, agricultural and medical processes. Psychrotrophic microbes could be valuable in agriculture as bio-inoculants and biocontrol agents for low temperature habitats. The use of psychrophiles as biofertilizers, biocontrol agent and bioremediators would be of great use in agriculture under cold climatic conditions.

For more open access journals in juniper publishers please click https://juniperpublishers.com/

For more articles on Advances in Biotechnology & Microbiology Please click on https://juniperpublishers.com/aibm/

Advances in Biotechnology & Microbiology in Full text in Juniper Publishers

https://juniperpublishers.com/aibm/AIBM.MS.ID.555584.php

#Juniper Publishers #juniper publishers Reviews #Juniper Journals #Juniper Publishers in USA

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kocuria · 1 year

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“You look so perfect when you’re blissed out. I can never get enough of seeing you like that.”

for chapter 11 of A Call to Motion by JJK (@trenchcoatsandtimetravel) 😇 (93k, E)

my art ✵ commissions ✵ Twitter

#marvel #marvel fanart #stucky #stucky fanart #stevebucky #stevebucky fanart #captain america #captain america fanart #steve rogers #steve rogers fanart #bucky barnes #bucky barnes fanart #jjk #kocuria visuals #thirsty #sof'tato #Bucky's hair #kocuria original

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madamlaydebug · 7 years

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More Than A Primitive Practice: How Smudging Kills Bacteria In The Air By Matt Caron Smudging is one of the most timeless practices in the world. Believe it or not, it actually dates back to prehistoric times! Obviously, it’s still used to this day to clear negative energy — though many people think this is complete woo-woo. But recent scientific discoveries has revealed that it actually has a powerful anti-bacterial effect. The scientific paper entitled “Medicinal Smokes” and published in the Journal of Ethnopharmacology focuses a scientific lens on the practice, which is becoming more and more widely used, despite skepticism. The study looked into herbal and non-herbal remedies that were administered by the burning of various plant matter. The research included information from 50 countries and found that, predominantly, smoke administered medicinally is mostly used to aid lung, brain and skin function. It was also said that passive fumes doubled as a sort of air purifier. The purpose of the study was to see whether or not medicinal smoke deliveries could be explored by western medicine, because “The advantages of smoke-based remedies are rapid delivery to the brain, more efficient absorption by the body and lower costs of production.” A follow-up paper found that the research concluded that, in addition to health benefits, smudging was a powerful antiseptic: “We have observed that 1 hour treatment of medicinal smoke emanated by burning wood and a mixture of odoriferous and medicinal herbs (havan sámagri=material used in oblation to fire all over India), on aerial bacterial population caused over 94% reduction of bacterial counts by 60 min and the ability of the smoke to purify or disinfect the air and to make the environment cleaner was maintained up to 24 hour in the closed room. Absence of pathogenic bacteria Corynebacterium urealyticum, Curtobacterium flaccumfaciens, Enterobacter aerogenes (Klebsiella mobilis), Kocuria rosea, Pseudomonas syringae pv. persicae, Staphylococcus lentus, and Xanthomonas campestris pv. tardicrescens in the open room even after 30 days is indicative of the bactericidal potential of the medicinal smoke treatment. We have demonstrated that using medicinal smoke it is possible to completely eliminate diverse plant and human pathogenic bacteria of the air within confined space.” Shockingly, burning herbs such as sage cleared bacteria by 94%! That is truly incredible. A day later, the space was still found to be disinfected. A month later, many of the original pathogens were still undetectable. Think about this. It’s incredible news! Modern air quality is atrocious in many parts of the world. This could be an excellent way to mitigate those effects. Smudging is much better than using a chemical storm to disinfect an area, and even last longer. Amazing!

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ao3feed-buckybarnes · 4 years

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Time is a Metaphor

read it on the AO3 at https://ift.tt/39ucwAP

by CrushedRose, kocuria

Time is a wonderful teacher, but in the end, kills all his students.

Words: 4960, Chapters: 1/44, Language: English

Series: Part 3 of The Value of Time

Fandoms: Captain America (Movies), The Avengers (Marvel Movies)

Rating: Teen And Up Audiences

Warnings: No Archive Warnings Apply

Categories: Multi

read it on the AO3 at https://ift.tt/39ucwAP

#bucky #IFTTT #ao3feed #fanfic #bucky barnes #james barnes #the winter soldier #james barnes

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healthspiritbody · 3 years

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Research Shows That “Smudging” Works To Eliminate Dangerous Bacteria in the Air

Mystics say the Native American practice of smudging or purifying a room with the smoke of sacred herbs, can help clear negative energy from a space. Now, modern scientific research reveals that the practice may actually have health-benefiting implications by purifying the air of harmful bacteria.

The burning of herbs and plant resins for medicinal and spiritual purposes, aka ‘smudging’, is an ancient practice among indigenous people around the world; recently adopted by Westerners at an astonishing rate. Smudging is believed to unlock the ‘spirits’ of various plant allies to restore balance and ease to an individual or group.

Many views smudging as “taking a spiritual shower” — ridding those that have adopted the practice of the negative energy that follows them. On the contrary, skeptics believe attributing health benefits to the burning of sage and incense reflects ‘magical thinking.’ The practice has even been accused of being a New Age form of cultural imperialism where ‘plastic’ or ‘white’ shamans mimic and co-opt the traditions of indigenous people their predecessors essentially conquered.

In a 2006 article titled “Medicinal Smoke” published in the Journal of Ethnopharmacology, reviewers assessed multi-ingredient herbal and non-herbal remedies administered as smoke from 50 countries across 5 continents. The researchers found that medicinal smoke is largely utilized for the same reason worldwide — to address issues with specific organ systems: “pulmonary (23.5%), neurological (21.8%) and dermatological (8.1%).” They also found that “ambient smoke” (passively inhaled smoke generated by smudging/incense), is traditionally believed to be an effective “air purifier.” Given the findings, researchers argued that modern medicine should investigate medicinal smoke as a drug delivery system stating that, “the advantages of smoke-based remedies are rapid delivery to the brain, more efficient absorption by the body and lower costs of production.”

Smudging, in its traditional sense, is not aimed at ‘killing germs,’ or becoming a ‘new drug delivery system’ as the Journal of Ethnopharmacology article implies, but because modern biomedicine only acknowledges what is empirically verifiable – which does not include ‘the soul’ or ‘negative vibes’ – the practice will only attain a modicum of credibility from the perspective of the dominant, scientism-contaminated worldview, if it can be demonstrated that it actually performs a useful function, such as destroying disease-causing germs.

Thanks to a groundbreaking 2007 study published in the Journal of Ethnopharmacology titled, “Medicinal smoke reduces airborne bacteria,” we now know that medicinal smoke has the potential to disinfect air by eliminating 94% of airborne bacteria in just 60 minutes. This bacteria includes staphylococcus lentus, Corynebacterium urealyticum, Curtobacterium flaccumfaciens, Enterobacter aerogenes (Klebsiella mobilis), Kocuria rosea, and more.

We have observed that treatment with medicinal smoke originated by a combination of burning wood, odoriferous, and medicinal herbs (havan sámagri=material used in oblation to fire all over India) for 60 minutes reduced 94% of airborne bacterial counts, and the air purifying abilities were retained for up to 24 hours in a closed room environment.

Furthermore, the absence of pathogenic bacteria Corynebacterium urealyticum, Curtobacterium flaccumfaciens, Enterobacter aerogenes (Klebsiella mobilis), Kocuria rosea, Pseudomonas syringae pv. persicae, Staphylococcus lentus, and Xanthomonas campestris pv. tardicrescens in the open room 30 days post treatment serves as a testament to the bactericidal potential of the medicinal smoke treatment.

Modern urban air has been found to contain at least 1800 diverse bacterial types – including families with pathogenic members – so the positive air-purifying results presented in the aforementioned paragraphs could have profound implications for combating an increasingly deadly array of antibiotic-resistant bacteria against which even the CDC itself has acknowledged its impotence. Consider also that a recent microbiome of NYC’s subway system found close to 1700 different microbes, including those responsible for Anthrax (Bacillus anthracis) and Bubonic Plague (yersinia pestis).

Also, considering that conventional chemical methods of air sterilization and odor neutralization (e.g. Lysol) are much less effective than advertised (one study found them up to 10 times less effective than believed), smudging or the use of natural incense products might constitute a far safer and more effective approach.

Given the aforementioned, one might infer that we as a society should start embracing medicinal smoke’s potent cleansing properties on aerosol microbes via Smudging as a real antiseptic rather than just a wives tale to cleanse negative energy.

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Research Shows That “Smudging” Works To Eliminate Dangerous Bacteria in the Air was originally published on Health Spirit Body

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